GATA3+Treg细胞在变应性鼻炎中的表达及意义  

Changes in percentage of GATA3+regulatory T cells and their pathogenic roles in allergic rhinitis

作　　者：孙柳 焦沃尔 孔勇刚[2] 杨长亮 续珊 谯月龙 陈始明[2] SUN Liu;JIAO Woer;KONG Yonggang;YANG Changliang;XU Shan;QIAO Yuelong;CHEN Shiming(Department of Otolaryngology,Head and Neck Surgery,General Hospital of central Theater Command,Wuhan 430070,China;Department of Otolaryngology Head and Neck Surgery,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]中部战区总医院耳鼻咽喉头颈外科,湖北武汉430070 [2]武汉大学人民医院耳鼻咽喉头颈外科,湖北武汉430060

出　　处：《南方医科大学学报》2023年第2期280-286,共7页Journal of Southern Medical University

基　　金：国家自然科学基金(81770981);湖北省自然科学基金(2022CFB437);中部战区总医院育英计划资助(ZZYCZ202105)。

摘　　要：目的 研究GATA3+Treg细胞在变应性鼻炎(AR)患者和小鼠中的表达和意义。方法 收集6例对照组患者(Control组)和6例变应性鼻炎患者(AR组)的鼻黏膜,检测其鼻黏膜炎症反应。同期收集12例Control组和12例AR组患者外周血单个核细胞(PBMC),检测其Treg细胞和GATA3+Treg细胞比例。将C57BL/6小鼠分为正常对照组(Control组)和变应性鼻炎组(AR组)。比较两组小鼠AR症状学评分、外周血OVA-sIgE水平、鼻黏膜炎症反应。收集小鼠脾脏,检测两组小鼠体内Treg细胞和GATA3+Treg细胞比例以及Th2细胞因子表达。结果 与Control组相比,AR组患者鼻黏膜中嗜酸性粒细胞浸润和杯状细胞增生明显增多(P<0.01),PBMC中Treg细胞和GATA3+Treg细胞表达下降(P<0.05)。动物实验发现:与Control组相比,AR组小鼠过敏症状评分、外周血OVA-sIgE水平、鼻黏膜嗜酸性粒细胞浸润和杯状细胞增生均明显增加(P<0.01)。与Control组相比,AR小鼠Treg细胞和GATA3+Treg细胞表达下调(P<0.01),并且Th2细胞因子IL-4、IL-6、IL-10表达上升(P<0.05)。结论GATA3+Treg细胞在AR患者和小鼠中表达下降,并可能与Th2细胞免疫应答相关,两者共同参与了AR的发生发展。GATA3+Treg细胞有望成为AR免疫调控的新靶点。Objective To investigate the changes in percentage of GATA3+regulatory T(Treg) cells in patients with allergic rhinitis(AR) and mouse models. Methods The nasal mucosa specimens were obtained from 6 AR patients and 6 control patients for detection of nasal mucosal inflammation. Peripheral blood mononuclear cells(PBMC) were collected from 12 AP patients and 12 control patients to determine the percentages of Treg cells and GATA3+Treg cells. In a C57BL/6 mouse model of AR, the AR symptom score, peripheral blood OVA-sIgE level, and nasal mucosal inflammation were assessed, and the spleen of mice was collected for detecting the percentages of Treg cells and GATA3+Treg cells and the expressions of Th2 cytokines.Results Compared with the control patients, AR patients showed significantly increased eosinophil infiltration and goblet cell proliferation in the nasal mucosa(P<0.01) and decreased percentages of Treg cells and GATA3+Treg cells(P<0.05). The mouse models of AR also had more obvious allergic symptoms, significantly increased OVA-sIgE level in peripheral blood, eosinophil infiltration and goblet cell hyperplasia(P<0.01), markedly lowered percentages of Treg cells and GATA3+Treg cells in the spleen(P<0.01), and increased expressions of IL-4, IL-6 and IL-10(P<0.05). Conclusion The percentage of GATA3+Treg cells is decreased in AR patients and mouse models. GATA3+Treg cells possibly participate in Th2 cell immune response, both of which are involved in the occurrence and progression of AR, suggesting the potential of GATA3+Treg cells as a new therapeutic target for AR.

关 键 词：变应性鼻炎 TREG细胞 GATA3+Treg细胞 免疫调控 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]