呼出气冷凝液中肿瘤坏死因子-α、白细胞介素-1β与慢性阻塞性肺病患者肺功能的相关性  

Correlation between tumor necrosis factor-αand interleukin-1βin exhaled breath condensate and pulmonary function in chronic obstructive pulmonary disease

作　　者：杨伟煌 刘榕敏[1] 桂文婷 YANG Wei-huang;LIU Rong-min;GUI Wen-ting(The Fourth Afiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 511300,China)

机构地区：[1]广州医科大学附属第四医院,广东广州511300

出　　处：《中国热带医学》2023年第2期173-175,204,共4页China Tropical Medicine

摘　　要：目的探究慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)患者肺功能与呼出气冷凝液(exhaled breath condensate,EBC)中肿瘤坏死因子-α、白细胞介素-1β水平的相关性,为COPD诊治和疗效判定提供便利的方法依据。方法选取2020年8月—2022年2月就诊于广州医科大学附属第四医院呼吸科的81例COPD患者与40名健康对照者为研究对象。根据COPD患者状况分为急性加重期组41例和缓解期组40例。对所有参与者行肺功能检测、血清和EBC采集,并采用酶联免疫吸附法ELISA分析血清及EBC中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的含量水平,利用Pearson相关性分析方法进行相关性分析。结果COPD急性加重期患者EBC中的TNF-α和IL-1β水平分别为(5.16±0.18)pg/μL和(7.75±0.27)pg/μL,健康对照组分别为(2.66±0.31)pg/μL和(2.41±0.24)pg/μL,COPD缓解期组分别为(3.61±0.29)pg/μL和(3.17±0.38)pg/μL,COPD急性加重期组EBC中的TNF-α和IL-1β水平较高(F=9.451,8.217,P<0.001),血清检测与该结果一致。相关性分析提示EBC与血清中TNF-α和IL-1β的含量水平呈显著正相关(P<0.001),与肺功能呈显著负相关(P<0.001)。结论EBC中的TNF-α和IL-1β是COPD患者气道炎症的潜在生物标志物,EBC中的TNF-α和IL-1β检测能够有效评估COPD患者肺功能。Objective To explore the correlation between lung function in patients with chronic obstructive pulmonary disease(COPD)and tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)levels in exhaled breath condensate(EBC),and to provide a convenient methodological basis for the diagnosis and treatment of COPD and the determination of its efficacy.Methods A total of 81 COPD patients and 40 healthy controls were selected from the respiratory department of the Fourth Affiliated Hospital of Guangzhou Medical University from August 2020 to February 2022 as the research subjects.The COPD patients were divided into 41 cases in the acute exacerbation group and 40 cases in the remission group according to their status.All participants underwent lung function detection,venous blood and EBC collection,and the levels of TNF-αand IL-1βin EBC and venous blood were analyzed by enzyme-linked immunosorbent assay(ELISA),and correlation analysis was performed by Pearson correlation analysis method.Results The levels of TNF-αand IL-1βin EBC of in the acute exacerbation group,the healthy control group,the remission group were(5.16±0.18)pg/μL and(7.75±0.27)pg/μL,(2.66±0.31)pg/μL and(2.41±0.24)pg/μL,(3.61±0.29)pg/μL and(3.17±0.38)pg/μL,respectively.Compared with the healthy control group,the levels of TNF-αand IL-1βin EBC in the COPD acute exacerbation group were significantly higher than those in the healthy control group and the COPD remission group(F=9.451,8.217,P<0.001).Serum tests were consistent with this result.Correlation analysis showed that the levels of TNF-αand IL-1βin EBC were significantly positively correlated with the level of serum inflammation levels(P<0.001),while significantly negatively correlated with lung function(P<0.001).Conclusions TNF-αand IL-1βin EBC are potential biomarkers of inflammation in patients with COPD,and their detection can be used to effectively assess lung function in patients with COPD.

关 键 词：呼出气冷凝液 肿瘤坏死因子-Α 白细胞介素-1Β 慢性阻塞性肺疾病 

分 类 号：R521.1[医药卫生—内科学]