田基黄总黄酮调节酒精性肝损伤小鼠肠道菌群及降低内毒素的研究  

Effects of flavonoids of hypericum japonicum thunb flavone in regulating intestinal microflora and reducing endotoxin in alcohol-induced liver injury mice

作　　者：苏镶月 王海艳[2] 崔刚[1] 张波[1] 侯霞[1] 吴庆田[3] SU Xiang-yue;WANG Hai-yan;CUI Gang;ZHANG Bo;HOU Xia;WU Qing-tian(Jiamusi University Basic Medical College,Jiamusi,Heilongjiang 154000,China)

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154000 [2]德州市人民医院感染性疾病科 [3]佳木斯大学基础医学院微生态―免疫调节网络与相关疾病重点实验室

出　　处：《中国微生态学杂志》2023年第1期45-48,53,共5页Chinese Journal of Microecology

基　　金：黑龙江省自然科学基金(LH2020H003)。

摘　　要：目的 使用中药田基黄调节酒精性肝病(ALD)小鼠的肠道菌群,降低小鼠血中内毒素(LPS)的含量,改善小鼠的肝脏功能,从而减少酒精对肝脏的损伤、降低ALD的发病率达到临床辅助治疗ALD的目的。方法 80只昆明小鼠(雌雄各半),体质量26~37 g。正常饲养1周后,随机选取20只小鼠作为正常组,剩余小鼠作为酒精模型组,模型组小鼠灌胃56°北京红星二锅头白酒0.3 mL,2次/d,连续灌胃45 d,末次小鼠灌胃白酒后,禁食12 h但不禁水,于第46天早上随机选取模型组与正常组小鼠各10只,采用眼球采血法取小鼠血液,检测小鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及血LPS水平来验证模型。造模成功后模型组余下50只小鼠随机分为自然恢复组、丽珠肠乐组以及田基黄总黄酮低、中、高剂量干预组(田基黄总黄酮剂量分别为10、20、40 mg/mL),每组10只。分别于造模成功第0天、灌药7 d后,于无菌条件下取小鼠粪便对小鼠粪便中的大肠埃希菌、肠球菌、双歧杆菌和乳杆菌进行平板培养并计数。先称量小鼠体质量,采血检测小鼠血清ALT、AST活性及血LPS水平;最后处死小鼠取其肝脏称重,计算肝体比。结果 造模成功后模型组小鼠血清ALT、AST活性以及血LPS水平、肝体比升高,肠道菌群失衡。田基黄总黄酮治疗7 d后小鼠血清ALT、AST活性以及血LPS水平、肝体比降低,肠道中大肠埃希菌、肠球菌数量显著减少和乳杆菌、双歧杆菌数量显著升高。结论 田基黄总黄酮可有效降低ALD小鼠血清转氨酶活性及血LPS水平,调节肠道菌群失衡,改善酒精引起的小鼠肝脏损伤,对酒精性肝损伤发挥了一定的治疗作用。Objective To use hypericum japonicum as a clinical adjuvant treatment of alcoholic liver disease(ALD), reduce the content of endotoxin(LPS) in blood, and improve the liver function of mice, reduce the damage of alcohol to liver,reduce the incidence of ALD and achieve the purpose of of alcoholic liver disease. Methods A total of 80 Kunming mice(half male and half female) were given normal feeding for 1 week, then 20 mice were randomly selected as normal control group, with the remaining ones as alcohol model group. The model group was intragastrically given 0.3 mL 56° Beijing Erguotou liquor twice a day for consecutive 45 days. After the last intragastrical administration of liquor, the mice were forbidden to eat for 12 hours but allowed to drink water. 10 mice each in model group and normal control group were randomly selected on the morning of 46th day. The eyeball method was used to collect blood samples for detection of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST) and LPS to verify successful modeling. After that, the model group was randomly divided into natural recovery group, Lizhu Changle group and low-, medium-and high-dose intervention groups(the dosages of total flavonoids were 10 mg/mL, 20 mg/mL and 40 mg/mL, respectively)(n = 10). After0 day of modeling and 7 days of administration, Escherichia coli, Enterococcus, Bifidobacteria and Lactobacillus in the feces were cultured and counted under aseptic conditions. The mice were weighed;activities of serum ALT and AST and the content of blood LPS were detected. Finally, the livers of the mice were weighed and the liver body ratio was calculated.Result After modeling, the serum ALT, AST, LPS and hepatosomatic ratio of mice in model group increased;imbalance of intestinal flora was observed. After 7 days of drug treatment, the serum ALT, AST, blood LPS and hepatosomatic ratio decreased in the intervention group;the counts of Escherichia coli and Enterococcus decreased significantly, while those of Lactobacillus and Bifidobacteria inc

关 键 词：田基黄总黄酮 酒精性肝损伤 肠道菌群 血内毒素 

分 类 号：R37[医药卫生—病原生物学]