域发喷剂敷料对糠秕马拉色菌体外抑菌活性的实验研究  

Experimental study on the in vitro antibacterial activity of Medi hair spray dressing against Malassezia

作　　者：高瑞佳 刘士瑞[2] 孙瑾鹏 樊靖华[1] 李福秋[2] GAO Ruijia;LIU Shirui;SUN Jinpeng;FAN Jinghua;LI Fuqiu(Xi'an Central Hospital,Xi'an 710004,China;The Second Hospital of Jilin University,Jilin 130041,China;Dalian Dermatology Hospital,Dalian 116021,China)

机构地区：[1]西安市中心医院,西安710004 [2]吉林大学第二医院,吉林130041 [3]大连皮肤病医院,大连116021

出　　处：《中国真菌学杂志》2023年第1期19-23,41,共6页Chinese Journal of Mycology

摘　　要：目的采用琼脂平板扩散法(打孔法)和微量液基稀释法两种体外药敏实验方法,观察域发喷剂敷料及其主要成分(微生物源性抗菌肽)对头皮脂溢性皮炎常见的马拉色菌体外抑菌活性。方法不同浓度域发喷剂敷料、微生物源性抗菌肽为实验组,采乐洗剂(浓度2%酮康唑洗剂)、域发喷剂敷料空白溶剂为对照组,用琼脂平板扩散法(打孔法)和微量液基稀释法测试体外对马拉色菌的抑制作用,同时测定MIC(最小抑菌浓度)值。结果域发喷剂敷料、微生物源性抗菌肽、采乐洗剂均对马拉色菌有不同程度的体外抑制作用,其抑菌效果与药物浓度呈正相关。域发喷剂敷料的MIC值为0.977μL/mL、微生物源性抗菌肽的MIC值为3.907μL/mL。结论域发喷剂敷料安全性高,不易产生耐药性,副作用少,有较好地抑制马拉色菌作用,使用方便,可用于临床上头皮脂溢性皮炎的治疗。Objective Two in vitro drug sensitivity test methods,ager plate diffusion method(punch method)and microliquid-based dilution method,were used to observe the in vitro antibacterial activity of Medi hair spray dressing and its main components(Microbial antimicrobial peptide)against common Malassezia on the surface of seborrheic dermatitis lesions of the head.Methods Medi Hair Spray Dressing with different concentration,microbial antimicrobial peptide were used as the experimental group,and Caile lotion(concentration 2%ketoconazole lotion),blank solvent of Medi Hair Spray Dressing were used as the control group,and the diameters of the inhibition zone were compared in each group.The inhibitory effect on Malassezia in vitro was tested by agar plate diffusion method(punch method)and microdilution method,and the MIC(minimum inhibitory concentration)values were also determined.Results Medi Hair Spray Dressing and microbial antimicrobial peptide inhibited Malassezia to varying degrees in vitro,and the effect was positively correlated with drug concentration.Medi Hair Spray Dressing MIC was 0.977μL/mL;microbial antimicrobial peptide MIC was 3.907μL/mL.Conclusion Medi Hair Spray Dressing is safe,and has a better inhibition of Malassezia effect.

关 键 词：域发喷剂敷料 脂溢性皮炎 马拉色菌 微生物源性抗菌肽 体外抑菌 MIC 

分 类 号：R379.9[医药卫生—病原生物学]