基于重测序的烟草赤星病抗性关联SNP位点挖掘  

Mining of SNPs Associated with Tobacco Brown-Spot Resistance Based on Re-sequencing

作　　者：王文静[1] 王凤龙[1] 焦芳婵[2] 孙光军[3] 宋光龙 陈风雷[3] 王卫民[4] 吴晶晶 王晓强 Wang Wenjing;Wang Fenglong;Jiao Fangchan;Sun Guangjun;Song Guanglong;Chen Fenglei;Wang Weimin;Wu Jingjing;Wang Xiaoqiang(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,266101;Yunnan Academy of Tobacco Agriculture Sciences,Kunming,655021;Tobacco Department Guizhou Branch of China Tobacco Corporation,Guiyang,550025;Zhengjiang Zhongyan Industry Co.Ltd.,Hangzhou,310000)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]云南省烟草农业科学研究院,昆明650021 [3]中国烟草总公司贵州省公司,贵阳550004 [4]浙江中烟工业有限责任公司,杭州310000

出　　处：《分子植物育种》2023年第5期1475-1480,共6页Molecular Plant Breeding

基　　金：中国农业科学院创新团队经费项目(ASTIP-TRIC04);中国烟草总公司云南省公司项目(2019530000241001)和中国烟草总公司贵州省公司项目(201920)共同资助。

摘　　要：赤星病是烟草上的重要叶部病害,每年给烟草生产造成巨大损失,培育抗赤星病品种是防治烟草赤星病为害的根本措施,而对赤星病抗性关联遗传位点的发掘、鉴定和利用是培育抗赤星病烟草品种的关键基础。本研究利用抗赤星病的烟草材料‘Beinhart 1000-1’和加工品质好但不抗赤星病的烟草品种‘K326’,通过种间杂交和F_(1)代自交分离,创建了‘Beinhart 1000-1’和‘K326’的F_(2)代杂交分离群体。随后,通过赤星病抗病性鉴定,本研究在F_(2)代分离群体中选择了19株抗赤星病材料和19株感赤星病材料,分别进行了基因组DNA提取,并通过BSA法进行了基因组重测序。在重测序数据分析基础上结合表型鉴定结果,本研究鉴定出差异SNP位点约170万个,进行烟草赤星病抗性关联SNP位点分析,找到赤星病抗性相关基因10个,促进了烟草抗赤星病分子育种的发展。Brown-spot is an important leaf disease to tobacco, which causes huge yield loss on tobacco production annually. Development of brown-spot resistant cultivars is a great measure to reduce the yield reduction caused by this pathogen. Thus, identification and utilization of brown-spot resistance genes is of great value to tobacco resistance breeding. To identify brown-spot resistance molecular markers, in this study, we developed an F_(2) population with tobacco cultivars ’Beinhart 1000-1’, an important brown-spot resistant cultivar, and ’K326’, a brown-spot susceptible cultivar with good industrial processing qualities. And, 19 resistant lines and 19 susceptible lines were obtained by brown-spot resistance screening and were subjected to genomic re-sequencing. Then, marker-trait association analyses was carried out based on the phenotypic characteristics and the genomic re-sequencing data to identify SNPs associated with tobacco brown-spot resistance. These assays identified about 1.7 million differential SNPs and 10 genes associated with tobacco brown-spot resistance. Findings of this study are helpful for tobacco resistance breeding against brown-spot.

关 键 词：烟草赤星病 重测序 SNP 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]