温肾通督针法对抑郁症肾阳虚证模型大鼠相关神经递质及cAMP-RAF1信号通路的影响  

Effect of the warming kidney for dredging governor channel acupuncture method on related neurotransmitters and the cAMP-RAF1 signal pathway on depression with kidney yang deficiency syndrome model rats

作　　者：曹灵修 任路[1] CAO Lingxiu;REN Lu(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Department of Rehabilitation Medicine,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450057,China)

机构地区：[1]辽宁中医药大学,沈阳110847 [2]郑州大学第五附属医院康复医学科

出　　处：《北京中医药大学学报》2023年第1期141-148,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(No.2018YFC1704300);国家自然科学基金面上项目(No.82274665);河南省重点研发与推广项目(No.222102310491);辽宁省高等学校创新团队(No.辽教函[2018]479号);辽宁省特聘教授项目(No.辽教发[2015]153号)。

摘　　要：目的通过建立抑郁症肾阳虚证模型大鼠,探究温肾通督针法的温肾调神功效,揭示温肾通督针法温阳解郁的作用机制。方法将大鼠任意分为正常组、模型组、温针组、抑制剂组、温针+抑制剂组,每组各6只。除正常组外,其余各组采用氢化可的松激素注射(肌肉注射,25 mg/kg)结合慢性不可预见性温和刺激进行造模。温针组和温针+抑制剂组采用针刺和艾灸2种方式进行干预;温针+抑制剂组和抑制剂组在取材前24 h灌胃给药ESI-09[10 mg/kg,环磷酸腺苷(cAMP)-RAF1信号通路抑制剂]。造模及干预前后,观察大鼠一般情况,采用强迫游泳实验、水迷宫实验观察大鼠行为学变化。酶联免疫吸附测定法检测脑源性神经营养因子(BDNF)、5-羟色胺(5-HT)、多巴胺(DA)及去甲肾上腺素(NE)含量。蛋白质印迹法检测大鼠海马cAMP-RAF1信号通路中cAMP、RAS相关蛋白1(RAP1)和RAF1的蛋白表达。结果干预后,与正常组比较,模型组、温针组、抑制剂组和温针+抑制剂组的体质量均降低(P<0.01),模型组、抑制剂组的肛温均降低(P<0.01)。与模型组比较,抑制剂组的体质量、肛温降低(P<0.05,P<0.01),温针组、温针+抑制剂组的体质量、肛温升高(P<0.05,P<0.01)。干预后,与正常组比较,模型组、抑制剂组和温针+抑制剂组的强迫游泳不动时间均延长(P<0.01),温针组强迫游泳不动时间差异无统计学意义。与模型组比较,抑制剂组强迫游泳不动时间延长(P<0.01),温针组、温针+抑制剂组强迫游泳不动时间均缩短(P<0.05,P<0.01)。干预后,与正常组比较,模型组、温针组、抑制剂组和温针+抑制剂组平台潜伏期、平台区域穿梭次数、平台象限时间占比均降低(P<0.01)。与模型组比较,抑制剂组平台潜伏期、平台区域穿梭次数、平台象限时间占比均降低(P<0.01),温针组、温针+抑制剂组平台潜伏期、平台区域穿梭次数、平台象限时间占比均升高(P<0.01)�Objective By estabtishing depression with kidney yang deficiency syndrome model rats,to explore the effect of warming kidney for dredging governor channel acupuncture method on warming kidney and regulating spirit,and reveal the mechanism underlying the warming kidney for dredging governor channel acupuncture method.Methods The rats were randomly divided into the normal group,model group,warm needle group,inhibitor group,and warm needle combined with inhibitor group,with six rats per group.Except for the normal group,the other groups were given a hydrocortisone hormone injection(intramuscular injection,25 mg/kg)combined with chronic unpredictable mild stimulation for modeling.Acupuncture and moxibustion were used in the warm needle group and warm needle combined with inhibitor group.The rats in the warm needle combined with inhibitor group and inhibitor group were orally treated with ESI-09(cAMP-RAF1 signal pathway inhibitor)at a dose of 10 mg/kg 24 h before being sacrificed.Before and after modeling and intervention,the general conditions of rats were observed,and forced swimming and water maze tests were used to observe the behavioral changes in rats.The levels of brain-derived neurotrophic factor(BDNF),5-hydroxytryptophan(5-HT),dopamine(DA),and norepinephrine(NE)were determined by ELISA.Western blotting was used to detect the expression of cAMP,RAS-related protein 1(RAP1),and RAF1 proteins in the cAMP-RAF1 signal pathway.Results After intervention,compared with the normal group,the body weights of the model group,warm needle group,inhibitor group,and warm needle combined with inhibitor group were decreased(P<0.01),as were the anal temperatures of the model group and inhibitor group(P<0.01).Compared with the model group,the body weight and anal temperature of the inhibitor group were decreased(P<0.05,P<0.01),while those of the warm needle group and warm needle combined with inhibitor group were increased(P<0.05,P<0.01).After intervention,compared with the normal group,the forced swimming immobility time of the

关 键 词：抑郁症 肾阳虚 针灸 cAMP-RAF1信号通路 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]