经典名方阳和汤基准样品量值传递分析  

Analysis on quality value transfer of benchmark sample of classical prescription Yanghe Decoction

作　　者：张泽康 王昌海 赵玥瑛 张晴 席铖 张馨雨 孙萌[1] 董爽 杜守颖[1] 白洁[1] 陆洋[1] ZHANG Ze-kang;WANG Chang-hai;ZHAO Yue-ying;ZHANG Qing;XI Cheng;ZHANG Xin-yu;SUN Meng;DONG Shuang;DU Shou-ying;BAI Jie;LU Yang(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中草药》2023年第3期756-767,共12页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(82173989)。

摘　　要：目的建立经典名方阳和汤基准样品HPLC指纹图谱并对其指标性成分(5-羟甲基糠醛、桂皮醛、肉桂酸、芥子碱硫氰酸盐、甘草酸)进行含量测定,研究阳和汤基准样品量值传递规律,为复方制剂研制奠定基础。方法制备15批阳和汤基准样品,建立HPLC指纹图谱,明确并归属特征峰;对阳和汤基准样品中指标性成分进行含量测定,分析指标性成分从饮片-基准样品的传递规律。结果15批阳和汤基准样品指纹图谱相似度>0.83,标定了13个共有峰,并对各共有峰进行药材归属,其中2、8号峰来自熟地黄;10、11号峰来自肉桂;1、3~6号峰来自炒白芥子;13号峰来自麻黄;7、9、12号峰来自甘草。指标性成分5-羟甲基糠醛、桂皮醛、肉桂酸、芥子碱硫氰酸盐、甘草酸质量分数分别为0.028%~0.508%、0.076%~0.244%、0.006%~0.018%、0.430%~0.677%、0.100%~0.335%,转移率分别为1.21%~10.74%、2.68%~12.76%、11.46%~31.43%、39.61%~60.30%、4.90%~18.69%;基准样品出膏率为27.38%~40.73%。结论采用指纹图谱、出膏率及指标性成分含量测定相结合的模式,对经典名方阳和汤基准样品的量值传递过程进行分析,可初步拟定阳和汤基准样品的质量标准,为后期复方制剂的研究提供参考。Objective The HPLC fingerprint of the benchmark sample of classical prescription Yanghe Decoction(阳和汤)was established and the content of its index components(5-hydroxymethylfurfural,cinnamaldehyde,cinnamic acid,sinapine thiocyanate and glycyrrhizic acid)was determined to study the quantity transfer law of the benchmark sample of Yanghe Decoction,which laid the foundation for the development of compound preparation.Methods A total of 15 batches of Yanghe Decoction benchmark samples were prepared,fingerprints were established,and characteristic peaks were identified and assigned;The content of index components in the benchmark sample of Yanghe Decoction was determined,and the transfer law of index components from prepared pieces to benchmark samples was analyzed.Results The fingerprint similarity of 15 batches of Yanghe Decoction was more than 0.83,13 common peaks were calibrated,and the medicinal materials of each common peak were assigned,peaks 2 and 8 came from Shudihuang(Rehmanniae Radix Praeparata);Peaks 10 and 11 came from Rougui(Cinnamomi Cortex);Peaks 1,3—6 came from Chaobaijiezi(Sinapis Albae Semen Frictum);Peak 13 came from Mahuang(Ephedrae Herba),and peaks 7,9,12 came from Gancao(Glycyrrhizae Radix et Rhizoma).The mass fraction ranges of 5-hydroxymethylfurfural,cinnamaldehyde,cinnamic acid,sinapine thiocyanate and glycyrrhizic acid are 0.028%—0.508%,0.076%—0.244%,0.006%—0.018%,0.430%—0.677%and 0.100%—0.335%,respectively.The transfer rates range from 1.21%to 10.74%,2.68%to 12.76%,11.46%to 31.43%,39.61%to 60.30%and 4.90%to 18.69%,respectively;The extract rate of the benchmark sample ranges from 27.38%to 40.73%.Conclusion By using the mode of fingerprint,extract rate and index component content determination,the quantity transfer process of the classical prescription Yanghe Decoction benchmark sample was analyzed,and the quality standard of the benchmark sample of Yanghe Decoction could be preliminarily drawn.It can provide reference for the research of compound preparation in the later st

关 键 词：经典名方 阳和汤 基准样品 HPLC 指纹图谱 量值传递 5-羟甲基糠醛 桂皮醛 肉桂酸 芥子碱硫氰酸盐 甘草酸 

分 类 号：R283.6[医药卫生—中药学]