苯二甲酸异构体的RPLC保留行为和热力学性质  

Retention behavior and thermodynamic properties of phthalic acid isomers by RPLC

作　　者：张玉堂 陈海相[1] 李晓莲 李冲 郎巧文 ZHANG Yutang;CHEN Haixiang;LI Xiaolian;LI Chong;LANG Qiaowen(Key Laboratory of Advanced Textile Materials and Manufacturing Technology,Ministry of Education,Zhejiang Sci-Tech University,Hangzhou 310018,China)

机构地区：[1]浙江理工大学先进纺织材料与制备技术教育部重点实验室,杭州310018

出　　处：《现代纺织技术》2023年第2期80-85,共6页Advanced Textile Technology

基　　金：浙江省文物保护科技项目(2022001)。

摘　　要：在反相液相色谱(RPLC)中以C_(18)色谱柱为固定相,用15%~45%甲醇水溶液为流动相,研究了苯二甲酸异构体在10~50℃范围内的色谱保留行为和热力学性质。结果表明:邻苯二甲酸(PA)、对苯二甲酸(TPA)和间苯二甲酸(IPA)分子结构上的细微差异能在RPLC中清晰地体现出来。同时,根据苯二甲酸异构体与固定相之间的相互作用,测定了ΔH^(0)和ΔS^(0)的热力学参数变化。这些ΔH^(0)和ΔS^(0)的测定能为苯二甲酸异构体与疏水界面相互作用机制的研究提供一种行之有效的方法,也可为聚酯降解回收、生态环保、食品接触材料迁移物质等分析任务提供基础数据。Polyethylene terephthalate(PET), commonly known as polyester, is a condensation of terephthalic acid(TPA) and ethylene glycol(EG). In the process of PET production and processing, IPA and PA are widely used as important additives. In the process of production and use, the residual or degraded phthalic acid substances will seriously harm human health if they accidentally enter the human body. At home and abroad, no matter as packaging materials for food and medicine, or as fabrics for underwear, there are corresponding national standards for the residual or migratory amount of TPA, IPA and PA. Therefore, in the process of analysis and detection, it is necessary to be able to quickly separate the three and accurately quantify them.Reversed phase liquid chromatography(RPLC) has been widely used in chromatographic analysis because of its advantages such as small sample size, good separation and excellent repeatability. In order to further optimize the separation effect of benzoic acid isomers in liquid chromatography, we studied the chromatographic retention behavior of benzoic acid isomers in C18stationary phase with different proportions of mobile phase and different temperatures using methanol-aqueous solution as the mixed mobile phase, and calculated the thermodynamic parameters of the interaction between benzoic acid isomers and stationary phase. The stationary phase was C18column in RPLC, and the mobile phase was 15%~45% methanol aqueous solution. It was found that the Van’t Hoff curve and the lnk and Ψ had a good linear relationship in the temperature range of 10 to 50 ℃. In the process of binding with C_(18) column fixation, the binding mechanism did not change, and the retention behavior of terephthalic acid isomers controlled by mobile phase composition and column temperature was continuous and stable. Both ΔH^(0) and ΔS^(0) are negative values, indicating that the benzoic acid isomers are bound, the degree of freedom decreases, and energy is released during the process of C_(18) column fixation. Und

关 键 词：苯二甲酸异构体 反相液相色谱 保留行为 热力学参数 

分 类 号：O657.7[理学—分析化学]