漆酶/TEMPO体系催化氧化处理对黄麻纤维木质素的影响  

Effects of oxidative treatment catalyzed by the laccase/TEMPO system on lignins in jute fibers

作　　者：刘玲[1,2] 徐帅 王汇锋[3] 董爱学 L Liuling;XU Shuai;WANG Huifeng;DONG Aixue(Yancheng Polytechnic College,Yancheng 224005,China;Jiangsu Province Engineering Research Center of Biomass Functional Textile Fiber Development and Application,Yancheng 224005,China;Biwei Shenyou Quality and Technical Service Jiangsu Co.,Ltd.,Wuxi 214430,China;Key Laboratory of Eco-Textiles,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]盐城工业职业技术学院,江苏盐城224000 [2]江苏省生物质功能纺织纤维开发与应用工程研究中心,江苏盐城224000 [3]必维申优质量技术服务江苏有限公司,江苏无锡214430 [4]江南大学生态纺织教育部重点实验室,江苏无锡214122

出　　处：《现代纺织技术》2023年第2期185-190,共6页Advanced Textile Technology

基　　金：2022年江苏省科技厅产学研合作项目(BY2022192,BY2022421);2021年盐城工业职业技术学院横向课题(2021HX-75);中国博士后科学基金面上资助项目(2021M701452)。

摘　　要：采用漆酶/TEMPO体系对黄麻纤维进行催化氧化处理,通过Klason法测定处理前后黄麻纤维中的木质素含量,分别利用凝胶渗透色谱法、有机元素分析法、核磁共振氢谱法测定以二氧六环法从黄麻纤维中提取木质素的分子量及分布、元素含量与化学结构。结果表明:经漆酶/TEMPO处理后黄麻纤维中木质素含量由10.83%降为8.59%,纤维上存留的木质素亦发生了降解,重均分子量由439938 Da降至238704 Da。黄麻纤维经过漆酶/TEMPO体系处理后,木质素的碳元素含量升高,氧元素和—OCH_(3)含量降低,醇羟基数目减少,酚羟基数目稍有下降,并且结构单元的连接方式有所转变,其中β-β、β-1和β-O-4结构比例有所降低,β-5结构增多,说明漆酶/TEMPO催化黄麻纤维木质素发生氧化反应并伴随有一定脱甲基化作用,木质素降解以β-β、β-O-4和β-1键断裂为主,而后形成β-5连接。In order to improve the spin ability and the added value of jute fibers, it is necessary to refine them. However, traditionally, lkali cooking and oxidation bleaching are mainly adopted to remove impurities such as lignin, but the environment pollution is serious. Biological refinement has attracted wide attention due to its high specificity of enzyme action, mild reaction conditions and environmental protection, but its impurity removal rate is low. The chemical–biological combination method is to combine the two, so that it has the advantages of thorough refinement of the chemical method and little fiber damage of the biological method.In order to remove impurities such as lignin in jute fibers, the study was based on the fact that laccase, as a copper-containing polyphenol oxidase, can form free radicals by catalytic oxidation with the phenolic hydroxyl group contained in the molecular structure of lignin through laccase, thus triggering degradation, polymerization or grafting reactions of lignin. Based on the mechanism of chemical structure change of lignin in jute fibers after laccase/TEMPO catalytic oxidation treatment, the laccase/TEMPO system was first used for catalytic oxidation of jute fibers, and then the lignin content in jute fibers before and after treatment was determined by the Klason method. The molecular weight, distribution, element content and chemical structure of lignin extracted from jute fibers by the dioxane ring method were determined by gel permeation chromatography, organic element analysis and nuclear magnetic resonance spectrometry. In this paper, we innovated the laccase/TEMPO oxidation system to treat jute fibers, and studied the changes of lignin content, molecular weight, chemical structure and functional groups in jute fibers, revealing the treatment mechanism of jute fibers by the oxidation system.The results showed that the lignin content in jute fibers decreased from 10.83% to 8.59% after the laccase/TEMPO treatment. The lignin remaining on jute fibers was also degraded, w

关 键 词：黄麻纤维 木质素 漆酶 TEMPO 氧化 

分 类 号：TS192[轻工技术与工程—纺织化学与染整工程]