化学同位素标记-质谱分析技术在环境健康研究中的最新进展  

Recent Progresses of Chemical Isotope Labeling-based Mass Spectrometry Analysis in Environment and Health Studies

作　　者：朱树芸[1,2] 高硕 周晓璐 赵先恩[1,2] ZHU Shu-yun;GAO Shuo;ZHOU Xiao-lu;ZHAO Xian-en(School of Chemistry and Chemical Engineering,Qufu Normal University,Qufu 273165,China;Key Laboratory of Life-Organic Analysis of Shandong Province,Qufu Normal University,Qufu 273165,China)

机构地区：[1]曲阜师范大学化学与化工学院,山东曲阜273165 [2]曲阜师范大学,山东省生命有机分析重点实验室,山东曲阜273165

出　　处：《质谱学报》2023年第2期197-212,共16页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金面上项目(22176109,22076097)。

摘　　要：代谢组和暴露组的分析表征在环境风险和健康效应研究中具有重要意义。由于人体代谢物和外源性化合物的理化性质多样和样品基质复杂,对其进行综合分析与表征是一项巨大的挑战。质谱(MS)虽然具有强大的定性和定量能力,但存在对一些分析物的灵敏度、特异性等不足的问题。化学同位素标记(CIL)技术与MS结合不仅可以提高检测灵敏度,而且在特异性、准确度、组学覆盖率和分析通量等方面有着显著的优势。近年来,化学同位素标记-质谱(CIL-MS)技术广泛应用于与环境和健康相关的代谢物和暴露标志物的靶向或非靶向分析。本文综述了近3年来CIL-MS在含氨基、羧基、羰基、羟基和巯基的代谢物/外源性化合物研究中的最新进展,关注与其相关的环境风险与健康效应,并总结CIL技术在MS分析中的优势、最新动态以及存在的不足,展望CIL-MS技术的发展趋势。With the development of environmental and health research,the relationship between pollutants exposure and human health effects has become an important research field in recent years.The analysis of metabolome and exposome is of great significance in the studies of environmental risk and health effect.Both metabolomics and exposomics require to deeply analyze the characterization of small molecule metabolites and exogenous compounds in living organisms.Due to the diversity of physicochemical properties of metabolites and xenobiotics and the complexity of sample matrix,their comprehensive analysis and characterization are still a great challenge.Mass spectrometry(MS)has powerful qualitative and quantitative capabilities,but it still lacks sensitivity and specificity for some analytes.Chemical derivatization can introduce pre-designed and synthesized chemical groups through chemical labeling reaction,which can significantly change the molecular physicochemical properties of analytes,so as to improve the analytical effect.Although the use of isotope internal standard(ISs)can effectively correct the results of mass spectrometry,ISs have the disadvantages of difficult to synthesize and expensive,and can be purchased in extremely limited types and amounts.To solve the above problems,chemical isotope labeling(CIL)technique has been developed and applied to MS-based metabolome and exposome analysis.Chemical isotope labeling-based mass spectrometry(CIL-MS)is one of the important solutions for comprehensive analysis of metabolome or exposome.In its workflow,biological samples are derivatized using CIL reagents that target specific chemical functional groups.Metabolites or xenobiotics containing corresponding targeted functional groups react with CIL reagents to produce labeled products.CIL-MS can not only improve sensitivity,but also has advantages of specificity,accuracy,omics coverage and analytical throughput.In recent years,CIL-MS technique has been increasingly used for targeted or non-targeted analysis of metabolites

关 键 词：化学同位素标记-质谱(CIL-MS) 衍生化 环境分析 健康效应 综述 

分 类 号：O657.63[理学—分析化学]