基于小型离子阱质谱快速检测抗生素  

Rapid Detection of Antibiotic Pollutants Using Miniature Ion Trap Mass Spectrometry

作　　者：徐楚婷 阮慧文 成晓亮 张伟 徐强 聂文彬 王卫国[2,4] 李海洋 XU Chu-ting;RUAN Hui-wen;CHENG Xiao-liang;ZHANG Wei;XU Qiang;NIE Wen-bin;WANG Wei-guo;LI Hai-yang(University of Chinese Academy of Sciences,Beijing 100049,China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;Nanjing Qlife Medical Technology Co.,Ltd,Nanjing 211500,China;Jinkai Instrument(Dalian)Co.,Ltd,Dalian 116085,China)

机构地区：[1]中国科学院大学,北京100049 [2]中国科学院大连化学物理研究所,辽宁大连116023 [3]南京品生医疗科技有限公司,江苏南京211500 [4]金铠仪器(大连)股份有限公司,辽宁大连116085

出　　处：《质谱学报》2023年第2期259-266,共8页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金委重大研发计划(91961124,22027804);辽宁省自然科学基金(2021-MS-015);大连市重点科技研发计划项目(2022YF12GX020);中国科学院科研仪器设备研制项目(ZDKYYQ20210005);云南省刑事科学技术重点实验室开放课题(YJXK001)。

摘　　要：近年来,抗生素滥用导致的环境污染和健康风险引起广泛关注。小型离子阱质谱具有灵敏度高、检测速度快、便携等优点,可实现对目标物的高选择性、高通量检测。本文基于丙酮辅助光电离源小型离子阱质谱,初步建立了医疗领域6种常见抗生素的快速检测方法。通过优化气流和进样时序参数,使用脉冲吹扫模式和卤素灯闪热热解吸提高热解吸效率,显著提高了高沸点抗生素的质谱信号强度。利用同位素内标开展抗生素的初步定量研究,并进行水中抗生素检测研究,整个检测过程可在1 min内完成。在1μL进样量时,基于信噪比(S/N)=3计算得到异烟肼(INZ)、乙硫异烟胺(ETN)、伏立康唑(VCZ)的检出限分别为636、160、35μg/L。该小型离子阱质谱仪便携、分析速度快、检测灵敏度高,有望应用于抗生素重点排放源的现场、高通量检测。Antibiotic misuse is becoming a worldwide public health issue due to serious risk to human health and environment.Residual antibiotics in environment mainly come from the wastewater from pharmaceutical factories,aquaculture,animal husbandry and hospitals.Antibiotics are included in the newly published List of Newly Controlled Pollutants(2022 Edition)(Draft for Comments).However,test standards are still lacking.Highly accurate results of the trace amounts of antibiotics distributed in the environment can be obtained by mass spectrometry coupled with chromatography.Nonetheless,sample pretreatment is time-consuming and expert-required.Compared with laboratory detection,ambient miniature mass spectrometry can realize on-site detection of the antibiotics without or with little pretreatment.On-site detection can be used for rapid qualitative and semi-quantitative detection,which can reflect the levels of water body pollution and accurately trace the pollutant sources.On-site detection also can provide technical support for the regulatory governance of antibiotics.But now no studies have been reported on the on-site detection of environmental antibiotics in the field.It is urgent to develop a rapid detection method for antibiotic residues in the environment to assess antibiotic pollution and provide support for regulation.In this paper,the detection of antibiotics was carried out based on flash desorption and acetone assisted photoionization source miniature ion trap mass spectrometry,the detection parameters of antibiotics were systematically optimized including gas flow and injection time sequence,and a rapid and high throughput detection method of antibiotics was established.The efficiency of thermal desorption was improved using pulse purging mode and halogen lamp flash thermal desorption,and the signal intensities of high boiling point antibiotics were significantly increased.Quantitative detection of antibiotics was realized by isotope internal standard,and preliminary detection of antibiotics in water was carried

关 键 词：抗生素 小型离子阱质谱 热解吸 丙酮辅助光电离源 痕量检测 

分 类 号：O657.63[理学—分析化学]