急性髓细胞白血病患者外周血ABO基因mRNA表达分析  

Analysis of ABO gene mRNA expression in peripheral blood of patients with acute myeloid leukemia

作　　者：周世航[1] 王霓[1] 刘铭[2] 范亚欣[1] ZHOU Shihang;WANG Ni;LIU Ming;FAN Yaxin(Dalian Blood Center,Dalian 116001,China;Department of Cell Biology,Dalian Medical University)

机构地区：[1]大连市血液中心,辽宁大连116001 [2]大连医科大学细胞生物学教研室

出　　处：《中国输血杂志》2023年第2期112-115,共4页Chinese Journal of Blood Transfusion

摘　　要：目的了解急性髓细胞白血病患者外周血ABO基因mRNA表达特点。方法下载TCGA数据库急性髓细胞白血病RNAseq数据和GTEx数据库中的全血RNAseq数据。使用R软件分析急性髓细胞白血病和GTEx全血标本ABO基因mRNA表达水平的差异,分析急性髓细胞白血病ABO基因mRNA表达与DNA甲基化、免疫浸润以及预后的关系。结果急性髓细胞白血病ABO基因mRNA表达水平(中位数:1.333,上四分位数:3.654,下四分位数:0.401)低于对照组(中位数:3.576,上四分位数:3.747,下四分位数:3.470),差异有统计学意义(P<0.001)。急性髓细胞白血病ABO基因mRNA表达水平与转录起始位点下游+82(r=-0.249,P<0.001)、+618(r=-0.268,P<0.001)、+1080(r=-0.105,P<0.001)以及+1409位点(r=-0.210,P<0.001)甲基化存在负相关关系,与B细胞、CD8 T细胞、细胞毒性细胞、浆细胞样树突状细胞、T细胞、T辅助细胞、中央记忆型T细胞、辅助性滤泡T细胞以及调节性T细胞的免疫浸润存在正相关关系(P<0.001)。急性髓细胞白血病ABO基因mRNA高表达患者的生存时间(中位生存时间:366 d,置信区间:304-731 d)和低表达患者的生存时间(中位生存时间:731 d,置信区间:335-1402 d)之间的差异无统计学意义(P>0.05)。结论急性髓细胞白血病患者外周血ABO基因mRNA表达是下降的,其与ABO基因第一内含子DNA甲基化以及免疫浸润有关,而与预后无关。Objective To investigate the expression characteristics of ABO gene mRNA in peripheral blood of patients with acute myeloid leukemia.Methods The RNA-seq data of acute myeloid leukemia in TCGA database and the whole blood RNA-seq data in GTEx database were downloaded.The difference of ABO gene mRNA expression between acute myeloid leukemia and GTEx whole blood samples was analyzed by R software,and the relationship between ABO gene mRNA expression and DNA methylation,immune infiltration and prognosis was analyzed.Results The expression level of ABO gene mRNA in acute myeloid leukemia(median:1.333,P25:3.654,P75:0.401)was significantly lower than that in the control group(median:3.576,P_(25):3.747,P_(75):3.470)(P<0.001).The expression level of ABO gene mRNA in acute myeloid leukemia was negatively correlated with the methylation of+82(r=-0.249,P<0.001),+618(r=-0.268,P<0.001),+1080(r=-0.105,P<0.001)and+1409(r=-0.210,P<0.001)at downstream of transcription start site,and positively correlated with the immune infiltration of nine types of immune cells(B cells,CD8 T cells,Cytotoxic cells,pDC,T cells,T helper cells,Tcm,Tfh and Treg)(P<0.001).There was no significant difference in overall survival between patients with high(median survival time:366 days,confidence interval:304-731 days)and low(median survival time:731 days,confidence interval:335-1402 days)expression of ABO gene mRNA in acute myeloid leukemia(P>0.05).Conclusion The mRNA expression of ABO gene in peripheral blood of patients with acute myeloid leukemia is decreased,which is associated with DNA methylation of the first intron of ABO gene and immune infiltration,but not with the prognosis.

关 键 词：急性髓细胞白血病 ABO基因 MRNA TCGA数据库 

分 类 号：R457.11[医药卫生—治疗学] R733.71[医药卫生—临床医学]