静注人免疫球蛋白中IgG二聚体含量对IgG Fc段与THP-1细胞表面受体结合能力的影响  

Effect of IgG dimer concentration on the binding ability of IgG Fc fragment in IVIG to THP-1 cell surface receptors

作　　者：雷婷婷 张巍 李长清[1] 叶生亮[1] 杜晞[1] 马莉[1] LEI Tingting;ZHANG Wei;LI Changqing;YE Shengliang;DU Xi;MA Li(Institute of Blood Transfusion,Chinese Academy of Medical Sciences&Peking Union Medical College,Chengdu 610052,China)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2023年第2期125-129,共5页Chinese Journal of Blood Transfusion

基　　金：国家药品监督管理局血液制品质量控制重点实验室开放性课题(KF2021011);中国医学科学院医学与健康科技创新工程项目(2021-12M-1-060)。

摘　　要：目的研究静注人免疫球蛋白(Intravenous immunoglobulin,IVIG)中免疫球蛋白G(Immunoglobulin G,IgG)二聚体含量对IgG Fc段与THP-1细胞表面受体结合能力的影响。方法利用蛋白纯化技术和高效液相色谱(HPLC)技术,制备不同浓度的IgG二聚体,然后采用直接添加的方式,制备得到含有不同浓度IgG二聚体的IVIG。最后利用本实验室已建立的IgG Fc段与THP-1细胞表面Fc段受体结合能力的检测方法,考察IgG二聚体含量对IgG Fc段与细胞表面受体结合能力的影响。结果当IVIG中IgG二聚体含量为1.11%~10.30%时,其与THP-1细胞表面Fc段受体的结合能力为97.67%~135.33%,且这种结合能力与IgG二聚体的含量呈正相关。当IgG二聚体含量超过13.22%时,IgG Fc段与THP-1细胞表面受体的结合能力与IgG二聚体含量则没有相关性。结论一定浓度的IgG二聚体对IVIG中IgG Fc段与THP-1细胞表面受体的结合能力具有促进作用,但尚需动物实验和临床数据进一步验证。Objective To investigate the effect of immunoglobulin G(IgG)dimer concentration of intravenous immunoglobulin(IVIG)on the binding ability of IgG Fc fragment to THP-1 cell surface receptors.Methods Firstly,protein purification and high performance liquid chromatography(HPLC)were used to prepare different concentrations of IgG dimers.After that,IgG dimer was added to IVIG to prepare IVIG containing different concentrations of IgG dimer.Finally,based on the method established in our laboratory,we analyzed the effect of IgG dimer concentration in IVIG on the binding ability of IgG Fc fragment to THP-1 cell surface receptors.Results When the concentration of IgG dimer in IVIG was 1.11%-10.30%,its binding ability to Fc receptors on the surface of THP-1 cell was 97.67%-135.33%,and this binding ability was positively correlated with the concentration of IgG dimer.When the IgG dimer concentration exceeded 13.22%,the binding ability had no correlation with the IgG dimer concentration.Conclusion A certain concentration of IgG dimer can promote the binding ability of the IgG Fc fragment in IVIG to receptors on the surface of THP-1 cells,which needs further verification from animal experiments and clinical data.

关 键 词：静注人免疫球蛋白(IVIG) IgG二聚体 IgG Fc段 THP-1细胞 受体结合能力 

分 类 号：R457.11[医药卫生—治疗学] R392.11[医药卫生—临床医学] R446.62