HBsAg单试剂反应性献血者检测结果追溯性分析  

Detection results of blood donors with HBsAg reactivity to single reagent:a retrospective analysis

作　　者：胡瑞 王婷婷 吕晴 王盼盼 李明睿 王婷 HU Rui;WANG Tingting;LYU Qing;WANG Panpan;LI Mingrui;WANG Ting(Anhui Blood Center,Hefei 230031,China)

机构地区：[1]安徽省血液中心,安徽合肥230031

出　　处：《中国输血杂志》2023年第2期164-167,共4页Chinese Journal of Blood Transfusion

摘　　要：目的对本中心初筛HBsAg ELISA单试剂反应性献血者血液标本进行追溯性检测分析,为进一步巩固献血者队伍提供依据。方法采集初筛HBsAg ELISA单试剂反应性献血者(已屏蔽至少6个月)的血液标本,进行HBsAg ELISA检测及NAT,同时采用罗氏电化学发光免疫分析法检测乙肝两对半,并对结果统计分析。结果51份初筛HBsAg ELISA单试剂反应性标本做追溯性检测,HBsAg双试剂均为阴性45份,单试剂呈反应性6份,追溯检测反应性率为11.76%(6/51)。NAT结果均为阴性。ECLIA法结果均为阴性,乙肝项5指标都为阴性的标本共23份,部分阳性28份,以抗-HBs、抗-HBc及抗-HBe阳性为主。结论通过对初筛HBsAg ELISA单试剂反应性标本的追踪检测,并补充检测HBV血清学标志物,可减少因检测而被屏蔽献血者数量,提高献血者再次献血的积极性,保护献血者权益。Objective To retrospectively analyze the detection results of blood donors with HBsAg reactivity to single reagent detected by enzyme-linked immunosorbent assay(ELISA)in our center,so as to provide basis for further consolidating the blood donor team.Methods Samples of blood donors who had been deferred for at least 6 months due to HBsAg reactivity to sole ELISA assay were collected,and HBsAg ELISA and NAT were further performed.Meanwhile,HBsAg/HBsAb/HBeAg/HBeAb/HBcAb were detected by Roche electrochemiluminescence immunoassay,and the results were statistically analyzed.Results Among these 51 selected samples,45 were negative to two assays,6 were reactive to sole assay,with reactivity-yield rate at 11.76%(6/51).The results of NAT/ECLIA were all negative.For five indicators of hepatitis B virus infection,23 samples were all negative and 28 were partially positive,mainly anti-HBs,anti-HBc and anti-HBe.Conclusion The follow-up detection of HBsAg ELISA sole-reagent reactive samples,supplemented with the detection of HBV serological markers,can reduce the number of deferred blood donors,increase the willingness to donate blood again,and protect the rights and interests of blood donors.

关 键 词：乙肝表面抗原 单试剂反应性 献血者 电化学发光免疫分析法 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2