改良白膜层离心装罐方法提高浓缩血小板质量的效果观察  

Observation on the effect of modified buffy coat centrifugal canning method on improving the quality of platelet concentrates

作　　者：罗曼华 康美艳 李津 吴宪彬 LUO Manhua;KANG Meiyan;LI Jin;WU Xianbin(Ganzhou Central Blood Station,Ganzhou 341000,China)

机构地区：[1]赣州市中心血站,江西赣州341000

出　　处：《中国输血杂志》2023年第2期193-195,共3页Chinese Journal of Blood Transfusion

基　　金：江西省卫生健康委科技计划项目(编号:202312127)。

摘　　要：目的对采用传统白膜层离心装罐方法和改良白膜层离心装罐方法制备浓缩血小板的质量进行评价,探讨改良白膜层离心装罐方法提高浓缩血小板质量的效果。方法将白膜层离心装罐方法分为传统组和改良组,传统组直接将白膜成分袋和空袋组合逐层贴合竖直装罐进行轻离心;改良组在白膜成分袋和空袋组合间添加离心隔板逐层贴合竖直进行轻离心,观察两组离心装罐方法对浓缩血小板制备质量的影响。结果采用改良白膜层离心装罐方法制备的浓缩血小板中血小板含量明显高于传统组,红细胞混入量低于传统组(P<0.05)。两组血小板含量合格率比较虽然没有统计学意义(P>0.05),但是改良组高于传统组,改良组红细胞混入量合格率高于传统组(P<0.05)。结论改良白膜层离心装罐方法可提升浓缩血小板质量,取材方便廉价,值得推广使用。Objective To evaluate the quality of platelet concentrates(PCs)prepared by traditional and modified buffy coat centrifugal canning methods,and to explore the effect of modified buffy coat centrifugal canning method on improving the quality of PCs.Methods The buffy coat centrifugal canning methods was divided into traditional group and modified group.In the traditional group,the buffy coat component bag and empty bag were directly combined layer by layer and vertically canned for light centrifugation.In the modified group,a foam spacer was added between the buffy coat component bag and the empty bag for light centrifugation layer by layer.The effects of the two groups of centrifugal canning methods on the preparation quality of PCs were observed.Results The platelet content in PCs prepared by the modified buffy coat centrifugal canning method was significantly higher than that in the traditional group,and the mixed amount of red blood cells was lower than that in the traditional group(P<0.05).Although there was no statistically significant difference in the qualified rate of platelet content between the two groups(P>0.05),the qualified rates of platelet content and mixed red blood cell in the modified group were higher than those in the traditional group(P<0.05).Conclusion The modified centrifugal canning method with buffy coat can improve the quality of PCs,which is convenient and cheap,and is worth popularizing.

关 键 词：浓缩血小板 装罐方法 离心隔板 白膜层 

分 类 号：R457.14[医药卫生—治疗学] R331.143[医药卫生—临床医学]