超声植冰协同海藻糖冷冻保存L-02肝细胞研究  

Cryopreserving L-02 hepatocytes with trehalose by ultrasonic ice seeding

作　　者：李维杰[1] 申敬 宋立勇 周新丽[1] 王建信 刘宝林[1,3] LI Weijie;SHEN Jing;SONG Liyong;ZHOU Xinli;WANG Jianxin;LIU Baolin(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Origincell Technology Group,Shanghai 201203,China;Co-innovation Center for Energy Therapy of Tumors,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]原能细胞科技集团,上海201203 [3]上海市肿瘤能量治疗技术与器械协同创新中心,上海200093

出　　处：《上海理工大学学报》2023年第1期50-56,共7页Journal of University of Shanghai For Science and Technology

基　　金：国家自然科学基金资助项目(51776130)。

摘　　要：采用海藻糖代替具有生物毒性的二甲基亚砜,利用自行搭建的超声植冰装置研究了超声植冰以及不同浓度海藻糖对L-02肝细胞冷冻保存的影响。结果表明,添加0.3 mol/L海藻糖结合超声植冰的细胞冻存组的肝细胞存活率较高((87.6±2.0)%),与传统植冰组((84.3±1.0)%)无显著性差异,与非植冰组((76.9±3.1)%)存在显著性差异。Effects of ultrasonic ice seeding and different concentrations of trehalose on the cryopreservation of L-02 hepatocytes were studied by replacing bio toxic dimethyl sulfoxide with trehalose in a self-built ultrasonic ice seeding device. The results show that the survival rate of hepatocytes(87.6±2.0)% is higher when cryopreserving L-02 hepatocytes with 0.3 mol/L trehalose by ultrasonic ice seeding, which is not significantly different from the traditional ice seeding group(84.3±1.0)%, and a significantly different from the non-ice seeding group(76.9±3.1)%.

关 键 词：超声植冰 海藻糖 肝细胞 冷冻保存 

分 类 号：R318.52[医药卫生—生物医学工程]