猫杯状病毒快速检测方法的建立  

Establishment of a Rapid Detection Method for Feline Calicivirus

作　　者：顾晏羽 钱刚 施桦千 赵庚彤 袁海宏 王睿涵 GU Yanyu;QIAN Gang;SHI Huaqian;ZHAO Gengtong;YUAN Haihong;WANG Ruihan(College of Animal Science and Food Engineering,Jinling Institute of Technology,Nanjing Jiangsu 210000,China)

机构地区：[1]金陵科技学院动物科学与食品工程学院,江苏南京210000

出　　处：《现代畜牧科技》2023年第3期17-19,共3页Modern Animal Husbandry Science & Technology

基　　金：大学生创新训练计划项目(202013573081CX);江苏省高等学校自然科学研究面上项目(20KJB230008);金陵科技学院高层次人才科研启动基金(jit-b-201912)

摘　　要：根据GenBank上公布猫杯状病毒(FCV)保守区域序列的6个区域,应用引物设计软件PrimerExplorerV5.0设计合成4条特异性引物,并对反应体系进行优化,建立用于检测FCV的环介导等温扩增(LAMP)方法。试验结果显示:该方法仅对FCV呈阳性反应,与猫泛白细胞减少症病毒、猫疱疹病毒、猫冠状病毒等其他常见猫传染病病原均无交叉反应,特异性强。与常规PCR方法检测相比,LAMP方法敏感性是常规PCR的100倍,敏感性强。对66份临床疑似病料样品分别采用新建立LAMP方法和常规PCR方法进行检测,结果显示,LAMP方法共检测出38例阳性样本,而常规PCR方法检测出29例。本研究建立一种猫杯状病毒的LAMP检测方法,具有简便快捷、灵敏度高等特点,能够用于FCV的临床快速检测。According to the 6 conserved regions of feline calicivirus(FCV)published in GenBank,four specific primers were designed and synthesized by PrimerExplorerV5.0,and the reaction system was optimized to establish a loop-mediated isothermal amplification(LAMP)method for detection of FCV.The results showed that the method was only positive for FCV,and had no cross reaction with other common feline infectious pathogens such as feline panleukopenia virus,feline herpes virus,feline coronavirus,and so on,which shows strong specificity.Compared with the conventional PCR method,the sensitivity of LAMP method is 100 times higher than that of conventional PCR.Sixty-six suspected clinical samples were detected by the newly established LAMP method and the conventional PCR method respectively.And the results showed that 38 positive samples were detected by LAMP method,while 29 cases were detected by the conventional PCR method.In conclusion,our study established a LAMP detection method for FCV,which is simple,rapid and sensitive,and can be used for rapid detection of FCVin clinical.

关 键 词：环介导等温扩增(LAMP) 猫杯状病毒(FCV) 可视化 

分 类 号：S858.293[农业科学—临床兽医学]