基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪  

Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR

作　　者：占建华 胡琴 朱勤俊 蒋滨 张许 刘买利 ZHAN Jianhua;HU Qin;ZHU Qinjun;JIANG Bin;ZHANG Xu;LIU Maili(State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan(Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences),Wuhan 430071,China;University of Chinese Academy of Sciences,Beijing 100049,China;Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China;Optics Valley Laboratory,Wuhan 430074,China)

机构地区：[1]波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院精密测量科学与技术创新研究院),湖北武汉430071 [2]中国科学院大学,北京100049 [3]华中科技大学,武汉光电国家研究中心,湖北武汉430074 [4]湖北光谷实验室,湖北武汉430074

出　　处：《波谱学杂志》2023年第1期22-29,共8页Chinese Journal of Magnetic Resonance

基　　金：国家重点基础研究发展计划资助项目(2017YFA0505400,2018YFE0202300,2018YFA0704002)。

摘　　要：细胞色素c(cytochrome c,cyt c)是一个重要的多功能蛋白.在线粒体中,它作为载体传递电子.而在细胞质中,它可能会作为凋亡起始因子启动细胞凋亡程序.复杂的细胞质环境是否会对其构象产生影响,以及产生怎样的影响,目前仍然没有得到确证.本研究通过无标记的基于甲基的核磁共振(nuclear magnetic resonance,NMR)技术追踪了野生型酿酒酵母iso-1 cyt c在酵母细胞匀浆液中的构象变化.发现cyt c在细胞匀浆中至少存在4种不同的氧化态构象和1种还原态构象.而且随着时间的推进,不同构象之间发生转换.结果表明cyt c的构象会随环境改变,这可能与抵抗氧化应激相关.Cytochrome c(cyt c)is an important multifunctional protein.In mitochondria,it acts as a carrier for electron transporting.In cytoplasm,it may act as an apoptotic initiator to initiate the apoptotic process.Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet.In this study,the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance(NMR)technique.At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate.And over time,the transitions among different conformations of cyt c were observed.The results indicate that the conformation of cyt c changes with environment,which may be closely related to resistance to oxidative stress.

关 键 词：核磁共振 构象变化 甲基 细胞色素C 

分 类 号：O482.53[理学—固体物理]