不同盐度、饵料和光照条件培养下拟伍氏游仆虫的种群动力学响应  

Effects of salinity,food,and light conditions on the population dynamics of Euplotes parawoodruffi

作　　者：吕湘琳 齐红莉 LV Xiang-lin;QI Hong-li(Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,College of Fisheries Science,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384

出　　处：《海洋科学》2023年第1期66-72,共7页Marine Sciences

基　　金：天津市自然科学基金项目(17JCYBJC29800、15JCYBJC23900);山东省重大科技创新工程专项“海洋药源生物基础研究”(2018SDKJ0406-4)。

摘　　要：为探究不同盐度、饵料和光照条件培养下拟伍氏游仆虫(Euplotes parawoodruffi)的种群动力学响应,本实验采用酵母和杜氏盐藻2种不同类型的饵料喂食拟伍氏游仆虫,在一系列盐度梯度(5、10、15、20、25)及2种光照条件[光照(14 L∶10 D)、全黑暗]下,测定了拟伍氏游仆虫的种群动力学参数变化。使用种群动力学参数(环境容纳量K、种群内禀增长率r、及世代时间G)评价盐度胁迫和不同培养条件对拟伍氏游仆虫的影响。结果表明:在盐度15处理组拟伍氏游仆虫的环境容纳量达到峰值,高盐度下(20~25)拟伍氏游仆虫的环境容纳量显著下降(P<0.05)。随盐度变化,杜氏盐藻组拟伍氏游仆虫的种群内禀增长率呈先上升后下降的趋势,酵母组种群内禀增长率呈先下降后上升的趋势;酵母组喂养的拟伍氏游仆虫环境容纳量高于杜氏盐藻喂养组,内禀增长率低于杜氏盐藻喂养组。光照条件下拟伍氏游仆虫的环境容纳量高于全黑暗条件,内禀增长率低于全黑暗条件。拟伍氏游仆虫对盐度胁迫的响应敏感,可作为环境监测的指示生物,在盐度为15,光照条件(14 L∶10 D)下使用酵母投喂,可实现拟伍氏游仆虫高密度的培养。This study aimed to investigate the effects of salinity stress and various culture conditions on the population dynamics of Euplotes parawoodruffi.Two different types of baits(Saccharomyces cerevisiae and Dunaliella salina)were used to feed E.parawoodruffi.Changes in population dynamics were evaluated under a series of salinity gradients(5,10,15,20,and 25)and two lighting conditions(14-h light∶10-h dark cycle and 24-h complete darkness).The population dynamics parameters carrying capacity(K),intrinsic growth rate(r),and generation time(G)were used to evaluate the impact of food and environmental elements on E.parawoodruffi.The carrying capacity reached peak levels at 15 salinity and decreased significantly(P<0.05)at higher salinity gradients(20 and 25).With increasing salinity,the intrinsic growth rate of E.parawoodruffi fed with D.salina increased initially and subsequently decreased,while the carrying capacity in the group fed with S.cerevisiae group decreased initially and then gradually increased.Additionally,E.parawoodruffi fed with S.cerevisiae exhibited a higher carrying capacity and lower intrinsic growth rate compared to the population fed with D.salina.Compared to 24 hours of complete darkness,a 14∶10 light-dark cycle increased the carrying capacity and decreased the intrinsic growth rate in E.parawoodruffi.In conclusion,a high-density culture of E.parawoodruffi can be achieved with S.cerevisiae feeding,15 salinity,and a 14∶10 light-dark cycle.

关 键 词：拟伍氏游仆虫 光照 盐度 饵料 种群生长 

分 类 号：S917.1[农业科学—水产科学]