Nitric oxide-generating self-assembling peptide hydrogel coating for enhancing hemocompatibility of blood-contacting devices  

作　　者：Hao Li Qianru Guo Qiufen Tu Kaiqin Xiong Wei Wang Lei Lu Wentai Zhang Nan Huang Zhilu Yang 

机构地区：[1]Key Laboratory of Advanced Technology for Materials of Education Ministry,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China [2]Affiliated Dongguan People’s Hospital,Southern Medical University,Dongguan 523059,China [3]State Key Laboratory of Molecular Engineering of Polymers(Fudan University),Shanghai 200438,China [4]Department of Cardiology,Zigong First People’s Hospital,Zigong 643000,China [5]School&Hospital of Stomatology,Wenzhou Medical University,Wenzhou 325035,China [6]Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs,School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China [7]Guangdong Provincial Key Laboratory of Shock and Microcirculation,Guangzhou 510080,China

出　　处：《Journal of Materials Science & Technology》2022年第36期106-114,共9页材料科学技术（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.82072072,32171326 and 31800795);the International Cooperation Project by the Science and Technology Department of Sichuan Province(No.2021YFH0056);the Sichuan Science and Technology Program(No.2021JDRC0160);the High-level Talents Research and Development Program of Affiliated Dongguan Hospital(No.K202102)。

摘　　要：Thrombosis is the major stumbling block to the clinical application of blood-contacting devices.Herein,a quick and easy surface engineering strategy of hydrogel coating with the therapeutic gas nitric oxide(NO)generation was reported to realize up-regulation of cyclic guanosine monophosphate(c GMP)and improve hemocompatibility for diverse metal materials.We first introduce the active centre selenocysteine of glutathione peroxidase(GPx)to the self-assembling peptide(RADA)4,obtaining a functionalized hydrogel.Then the hydrogel is directly coated on the 316L stainless steel(SS)for catalytically generating NO from endogenous s-nitrosothiols(RSNO).The generated NO endows the coated surface with regulation of platelet behavior and reduction of plasmatic coagulation activation and complement system activation,hence improving antithrombotic ability in vitro and ex vivo.Overall,our NO-generating hydrogel coating surface engineering strategy provides a novel solution to remove the obstacle about thrombosis of blood-contacting devices in clinic.

关 键 词：Blood-contacting device NO generation Self-assembling peptide Hydrogel coating Antithrombotic ability 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]