异育银鲫血清蛋白质图谱的构建及生物信息学分析  

Serum Proteins Profile and Bioinformatics Analysis of Silver Crucian Carp Carassius auratus gibelio

作　　者：皮乔木 王倩楠 刘有华 王伟霞 安贤惠 李福后 PI Qiaomu;WANG Qiannan;LIU Youhua;WANG Weixia;AN Xianhui;LI Fuhou(Jiangsu Key Laboratory of Marine Biological Resources and Environment,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005

出　　处：《水产科学》2023年第2期185-194,共10页Fisheries Science

基　　金：江苏省自然科学基金资助项目(BK20181484);江苏省研究生科研与实践创新计划项目(SJCX20_1289);江苏省优势学科建设工程资助项目(PAPD)。

摘　　要：异育银鲫是国内淡水养殖的优良品种之一,近年来,其病害问题日趋严重,极大影响了养殖效益。鱼类在感染病害后,可引起血清中某些蛋白质的表达水平发生变化,因此通过筛选某些特异蛋白质,将其作为生物标记物,有助于鱼类病害的识别及防治。利用蓝绿温和胶电泳结合色谱-质谱联用技术对异育银鲫血清蛋白质进行分离和鉴定,共鉴定成功721个血清蛋白质。生物信息学分析表明,这些蛋白质主要参与补体和凝血级联、代谢途径以及细菌感染途径等。本试验鉴定出多种非特异性免疫相关蛋白质,其中包含C3蛋白和α2-巨球蛋白,基于强度的绝对蛋白质定量分析表明,不同种类的补体C3蛋白和α2-巨球蛋白具有不同的表达量。部分潜在的血清标记物如血清转铁蛋白、肌酸激酶、α2-烯醇化酶以及补体因子Hf等在血清中均被成功鉴定。本试验结果可为异育银鲫血清蛋白质功能研究、蛋白质相互作用以及血清标记物筛选提供新的研究手段和方法,对于鱼类的疾病预防工作具有重要意义。Silver crucian carp Carassius auratus gibelio is one of the excellent varieties of freshwater aquaculture in China.In recent years,the disease problems are becoming more and more serious,and thus have greatly affected the breeding efficiency.Fish infected with diseases can cause changes in the expression levels of some proteins in serum.Therefore,screening some specific proteins as biomarkers is helpful to the identification and control of fish diseases.In the paper,the serum proteins of silver crucian carp were isolated and identified using blue-native polyacrylamide gel electrophoresis and LC-MS/MS.A total of 721 serum proteins were identified.Bioinformatics analysis showed that these proteins were mainly involved in complement and coagulation cascade,metabolic pathway and bacterial infection pathway.A lot of non-specific immunity related proteins including complement C3 proteins andα2-macroglobulin were identified.intensity-based absoluteprotein-quatification(iBAQ)analysis showed that different kinds of complement C3 proteins andα2-macroglobulin had different expression levels.Moreover,some potential biomarkers such as serum transferrin,creatine kinase,α2-enolase and complement factor HF were also determined.The finding provides a new method for the study of serum proteins function,protein-protein interaction and serum biomarker screening for silver crucian carp,which is of great significance for disease prevention of fish.

关 键 词：异育银鲫 血清 蛋白质组 生物信息学 

分 类 号：Q959.4[生物学—动物学]