厚唇裂腹鱼性腺发育周期及血清中性激素含量变化  

Gonadal Development and Changes in Concentration of Serum Sex Hormones in Schizothorax irregularis

作　　者：赵贺 赵年桦 李丽 强壮 聂竹兰[1,2,3] 魏杰 ZHAO He;ZHAO Nianhua;LI Li;QIANG Zhuang;NIE Zhulan;WEI Jie(College of Animal Science and Technology,Tarim University,Alaer 843300,China;State Key Laboratory Breeding Base for The Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Corps and The Ministry of Science and Technology,Alaer 843300,China;Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production&Construction Corps,Alaer 843300,China;School of Life Science and Technology,Tarim University,Alaer 843300,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]省部共建塔里木盆地生物资源保护利用国家重点实验室,新疆阿拉尔843300 [3]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [4]塔里木大学生命科学与技术学院,新疆阿拉尔843300

出　　处：《水产科学》2023年第2期233-240,共8页Fisheries Science

基　　金：国家自然科学基金资助项目(31560721,31860729);新疆生产建设兵团中青年科技创新领军人才计划项目(2018CB033);新疆维吾尔自治区研究生科研创新项目(TDGRI201918);中国海洋大学塔里木大学科研合作联合基金资助项目(ZHYLH201902)。

摘　　要：为探明厚唇裂腹鱼的精巢、卵巢周年发育及血清中性激素含量的变化,2020年8月—2021年8月,每月在克孜勒河采样,获得样本共计194尾,采用常规石蜡切片方法对其精巢和卵巢进行观察,以耳石为鉴定材料对厚唇裂腹鱼最小性成熟个体年龄进行研究,选取成熟鱼卵描绘卵径分布判断其产卵类型,采用酶联免疫法测定厚唇裂腹鱼各发育周期的雌二醇和睾酮的质量浓度。试验结果显示:厚唇裂腹鱼精巢和卵巢发育分为6个时期;雄鱼最小性成熟个体年龄为2龄,雌性最小性成熟个体年龄为3龄,精巢发育早于卵巢;厚唇裂腹鱼产卵期在3—5月,卵粒的数量百分比在直径2.9~<3.1 mm时出现峰值,产卵后的Ⅵ期卵巢中极少存在成熟的卵粒,判断其产卵类型为完全同步产卵类型;雌鱼与雄鱼血清中雌二醇和睾酮的质量浓度均在Ⅴ期时达到最高。厚唇裂腹鱼属于完全同步产卵类型,性腺于2017年3—5月发育成熟并产卵,雌二醇和睾酮的质量浓度在厚唇裂腹鱼繁殖期间显著增加。To investigate the annual development of testis and ovary of Schizothorax irregularis and the changes in sex hormone content in serum,samples were taken monthly in the Kizil River from August 2020 to August 2021,and a total of 194 samples were obtained.Their testis and ovary were observed by conventional histological method.The otoliths were used as identification material to determine the minimum age at sexual maturity of the fish.The eggs were selected to depict the distribution of egg diameter to determine the spawning type.The results showed that S.irregularis testis and ovary development was divided into six periods;the minimum age at sexual maturity was 2 years for males and 3 years for females,and testis development was earlier than ovary development;S.irregularis spawn from March to May,and the number percentage of egg peaked at 2.9—<3.1 mm in diameter,and very few mature eggs were present in the ovary at stageⅥafter spawning,which was judged to be a fully synchronized spawning type.The maximal serum concentrations of estradiol and testosterone were observed at stageⅤin both females and males.The findings showed that S.irregularis gonads matured and spawned from March to May 2017,and the concentrations of estradiol and testosterone were increased significantly during the reproduction of S.irregularis.

关 键 词：克孜勒河 厚唇裂腹鱼 性腺发育 性激素 

分 类 号：Q959.4[生物学—动物学]