初步探究LncRNA在甘蓝型油菜生态型分化中的作用  

Preliminary exploration of the role of LncRNA in the ecotype differentiation of Brassica napus L.

作　　者：杨太桦 杨福权 郜耿东 殷帅 金庆东 徐林珊 蒯婕 汪波[1] 徐正华[1] 葛贤宏[1] 王晶 周广生[1] YANG Tai-Hua;YANG Fu-Quan;GAO Geng-Dong;YIN Shuai;JIN Qing-Dong;XU Lin-Shan;KUAI Jie;WANG Bo;XU Zheng-Hua;GE Xian-Hong;WANG Jing;ZHOU Guang-Sheng(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China)

机构地区：[1]华中农业大学植物科学技术学院,湖北武汉430070

出　　处：《作物学报》2023年第5期1197-1210,共14页Acta Agronomica Sinica

基　　金：国家重点研发计划项目“大田经济作物优质丰产的生理基础与调控”(2018YFD1000900)资助。

摘　　要：甘蓝型油菜是我国重要的油料作物之一,根据其成花转变过程中对低温春化时间需求的不同分为3种生态型:春性、半冬性和冬性。前人研究发现,长链非编码RNA (LncRNA)可在多层面上调控基因的表达,参与对植物生长发育的调控。在拟南芥中, LncRNA可以通过调控春化途径相关基因的表达来影响开花。本研究以3种生态型甘蓝型油菜为材料,利用高通量测序技术进行苗期叶片的mRNA和LncRNA测序,初步探究LncRNA在油菜生态型分化及适应性形成中的作用。3种生态型甘蓝型油菜共差异表达基因的GO及KEGG富集分析表明,不同生态型甘蓝型油菜之间存在大量基础化合物合成代谢差异,特别是脂质类化合物。3种生态型甘蓝型油菜中共鉴定获得3775个LncRNA,其中285个在2个及以上生态型组合中存在差异表达,涉及到1517个候选靶基因。这些差异表达LncRNA涉及到的靶基因也富集到大量基础化合物合成代谢途径。通过mRNA-LncRNA联合分析,我们预测到了一个开花基因的调控网络,包含8个开花基因和23个LncRNA,涉及到温度和光信号调控通路。通过比较鉴定得到的LncRNA和972个甘蓝型油菜重要农艺性状QTL位置信息发现约90%的LncRNA位点和QTL区间存在重叠,且差异表达LncRNA和QTL的重叠位点在不同生态型油菜中的分布具有差异。结果说明LncRNA在油菜生态型分化及重要农艺性状形成中具有重要作用。Brassica napus L. is one of the important oil crops in China. Based on its different requirements for vernalization temperature during flower transition, Brassica. napus can be divided into three ecotypes(spring, semi-winter, and winter ecotype) .Previous studies found that long non-coding RNA(LncRNA) can regulate gene expression level in multiple levels and participate in the regulation of plant growth and development. In Arabidopsis thaliana, LncRNA can regulate flowering time by regulating the expression of genes related to vernalization pathway. In this study, to preliminarily explore the ecotype differentiation and adaptive formation among the three ecotypes of Brassica napus, three different ecotypes of Brassica. napus were used as the materials and high-throughput sequencing technology was used to sequence mRNA and LncRNA in seeding leaves. The GO and KEGG enrichment analysis of co-differential expression genes of three ecotypes of Brassica napus showed that there were a lot of differences in the anabolism of basic compounds, especially lipid compounds. A total of 3775 LncRNA sequences were identified from the three ecotypes of Brassica napus, among which 285 Lnc RNA were differentially expressed in two or three ecotype combinations, and 1517 candidate target genes were involved. The candidate target genes of these differential expression LncRNA were also enriched in large number of anabolism related pathways of basic compounds. Based on conjoint analysis of mRNA and LncRNA, we predicted a putative regulatory network in the flowering genes, included eight flowering time genes and 23 LncRNA,which were involved in the regulation of temperature and light signal pathways. By analyzing the location of QTLs for important agronomic traits in B. napus, we found that about 90% of LncRNA and QTLs intervals were overlapped. The distribution of different expression LncRNA and the location of QTLs were different in three ecotypes of Brassica napus, which suggesting that LncRNA played an important role in ecotype differ

关 键 词：甘蓝型油菜 生态型 长链非编码RNA 开花基因 农艺性状位点 

分 类 号：S565.4[农业科学—作物学]