小麦G2-like转录因子家族基因鉴定与表达模式分析  

Genome-wide identification and expression analysis of G2-like transcription factors family genes in wheat

作　　者：贾玉库 高宏欢 冯健超 郝紫瑞 王晨阳 谢迎新 郭天财 马冬云 JIA Yu-Ku;GAO Hong-Huan;FENG Jian-Chao;HAO Zi-Rui;WANG Chen-Yang;XIE Ying-Xin;GUO Tian-Cai;MA Dong-Yun(College of Agronomy,Henan Agricultural University/National Engineering Research Center for Wheat/Technology Innovation Center of Henan Wheat,Zhengzhou 450046,Henan,China)

机构地区：[1]河南农业大学农学院/国家小麦工程技术研究中心/河南省小麦技术创新中心,河南郑州450046

出　　处：《作物学报》2023年第5期1410-1425,共16页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2015BAD26B00);河南省科技攻关项目(212102110281)资助。

摘　　要：Golden2-Like(G2-like)转录因子,是属于MYB类转录因子中GARP超家族的成员,在调节叶绿体发育中起重要作用。本研究利用生物信息学方法对小麦G2-like基因进行了全基因组鉴定,并对其理化性质、亚细胞定位、启动子顺式作用元件及对非生物胁迫和激素的响应模式进行了分析。从小麦中共鉴定出87个G2-like基因,不均匀的分布在小麦21条染色体上,系统发育分析将这些基因分为14个亚族。蛋白质二级结构预测表明,小麦G2-like基因的氨基酸序列均以α螺旋和随机卷曲为主要结构。启动子顺式作用元件分析表明其上游2 kb区域含有7种(P-box、SpI、LTR、ABRE、MBS、TGA-element和AE-box)与逆境胁迫诱导相关顺式作用元件。其中Ta3AG2-like19含有顺式调控元件结合位点最多,一共有18个结合位点。qRT-PCR验证发现,Ta3AG2-like19、Ta3AG2-like20、Ta4AG2-like29和Ta6AG2-like52在PEG和盐胁迫下以及GA、IAA和ABA激素诱导下表达量显著上调,这些基因可能介导了小麦对多种非生物逆境的响应。Golden2-like(G2-like)transcription factor,a member of the GARP superfamily of MYB transcription factors,plays an important role in regulating chloroplast development.In this study,genome-wide identification of G2-like genes in wheat was carried out by bioinformatics methods,and their physicochemical properties,subcellular localization,cis-acting elements of promoters,and response patterns to abiotic stresses and hormones were analyzed.A total of 87 G2-like genes were identified from wheat,which distributed in evenly on 21 chromosomes in wheat.Phylogenetic analysis showed that these genes were divided into 14 subfamilies,and fragment replication was the main reason for the expansion of this gene family.The prediction of protein secondary structure revealed thatαhelix and random curl were the main amino acid sequences of G2-like gene in wheat.Promoter cis-acting elements showed that there were seven cis-acting elements(P-box,SpI,LTR,ABRE,MBS,TGA-Element,and AE-box)in 2-kb region upstream of the promoter.Among them,Ta3AG2-Like19 contained the most cis-regulatory element binding sites with a total of 18 binding sites.The qRT-PCR revealed.that the relative expression levels of Ta3AG2-like19,Ta3AG2-Like20,Ta4AG2-Like29,and Ta6AG2-Like52 were significantly up-regulated under PEG and salt stresses,and induced by GA,IAA,and ABA hormones.These genes may mediate the response of wheat plant to various abiotic stresses.

关 键 词：小麦 G2-like转录因子 生物信息学 逆境胁迫 表达分析 

分 类 号：S512.1[农业科学—作物学]