NaOH和壳聚糖对柚木种子发芽过程营养物质的动态影响  

Dynamic Effects of NaOH and Chitosan on Nutrients of Teak Seeds during Germination

作　　者：郑川玲 王慷林 李莲芳[2] 张青青[2] 杨永洁 杨历雨 沈松 吴俊多 刘进 杨博 ZHENG Chuanling;WANG Kanglin;LI Lianfang;ZHANG Qingqing;YANG Yongjie;YANG Liyu;SHEN Song;WU Junduo;LIU Jin;YANG Bo(Tangjiahe National Nature Reserve Management Office,Qingchuan 628100,Sichuan;Southwest Forestry University,Kunming 650224,Yunnan)

机构地区：[1]四川省唐家河国家级自然保护区管理处,四川青川628100 [2]西南林业大学,云南昆明650224

出　　处：《四川林业科技》2023年第1期52-58,共7页Journal of Sichuan Forestry Science and Technology

基　　金：国家“十三五”重点研发计划(2016YFD0600602-2)。

摘　　要：为了解NaOH浓度(%)、壳聚糖浓度(%)及浸种时间的水平及其组合对柚木(Tectona grandis)种子发芽过程中可溶性糖和淀粉的动态变化影响,采用L9(34)正交设计开展试验实施。分别于播种前(初始)和播种后7、14、21、28 d时测定以上2指标。结果,可溶性糖初始含量为1.39 mg·g^(-1),播种后4个阶段处理组合的可溶性糖分别为0.20~1.44、0.11~0.70、0.10~0.63和0.15~0.54 mg·g^(-1),播种后处理组合间可溶性糖含量呈现极显著(P<0.01)和显著(P<0.05,28 d时)的差异。种子淀粉初始含量为0.41 mg·g^(-1),播种后4个阶段处理组合其含量分别为0.14~2.16、0.35~0.90、0.44~0.68和0.32~1.01 mg·g^(-1),处理组合间除21 d的外,淀粉含量显著(P<0.05)或极显著(P<0.01,7 d时)的差异。壳聚糖浓度是影响可溶性糖含量变化的主导因子,有利于柚木种子可溶性糖生理活动的最优处理组合为3%NaOH浓度浸泡后0.2%壳聚糖浸泡12 h。NaOH浓度是影响淀粉含量变化的主导因子,促进淀粉含量增加的最优处理组合为5%NaOH浓度浸泡后0.2%壳聚糖浸泡18 h。In order to understand the effects of Na OH concentration(%), chitosan concentration(%) and soaking time on the dynamic changes of soluble sugar and starch during seed germination of Tectona grandis factorial levels and their treatment combinations(TCs) of, the L9(3~4) orthogonal design was employed to implement the experiment. The above two parameters were measured before sowing(initial) and at 7, 14, 21, 28 days after sowing. The results were as follows: the initial soluble sugar content was 1.39 mg·g^(-1), and other four stages of the TCs were 0.20-1.44, 0.11-0.70,0.10-0.63 and 0.15-0.54 mg·g^(-1), respectively. The soluble sugar content among the treatment combinations after sowing is extremely significant(P<0.01) and significant(P<0.05, 28 d). The initial seed starch content was 0.41 mg·g^(-1), and the contents of four treatment combinations after sowing were 0.14-2.16, 0.35-0.90, 0.44-0.68 and 0.32-1.01 mg·g^(-1),respectively. The starch content was significant(P<0.05) or extremely significant(P<0.01-7 d). The chitosan concentration was the dominant factor affecting the change of soluble sugar content, and the optimal TC facilitating physiological activity of soluble sugar of teak seeds was 0.2% chitosan solution presoaking seeds for 12 hours after 3%Na OH concentration solution soaking seed. Na OH concentration was the dominant factor affecting the changes of starch content, and the optimal TC to promote the increase of starch content was soaking in 5%Na OH concentration and then soaking in 0.2% chitosan for 18h.

关 键 词：柚木 NAOH 壳聚糖 可溶性糖 淀粉 

分 类 号：S792.99[农业科学—林木遗传育种]