5种培养基对蛋白核小球藻生长及其产物合成的影响  

Effects of Five Media on the Growth and Product Synthesis of Chlorella pyrenoidosa

作　　者：梁志波 刘平英 周有彩 陈必链[1,2] 何勇锦[1,2] LIANG Zhibo;LIU Pingying;ZHOU Youcai;CHEN Bilian;HE Yongjin(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China;Engineering Research Center of Industrial Microbiology of Ministry of Education,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建师范大学工业微生物教育部工程研究中心,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2023年第1期93-100,117,共9页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(32172997)。

摘　　要：以蛋白核小球藻CP-FJ9为出发藻株,在异养培养条件下,采用SE、HA-SK、BG11、Basal和BBM 5种培养基分别对小球藻CP-FJ9进行培养.通过测定生物量、比生长速率及细胞内主要成分,比较5种培养基对小球藻CP-FJ9生长及其产物合成的影响.实验结果表明:Basal培养基更适合小球藻CP-FJ9快速培养,可获得最高生物量12.93 g·L^(-1)和最大比生长速率0.64 d^(-1);而HA-SK培养基则更有利于蛋白质的合成,可获得最高蛋白质产量5.14 g·L^(-1).另外,在10 L发酵罐中使用HA-SK培养基异养培养蛋白核小球藻CP-FJ9,经72 h发酵后,小球藻最终生物量为20.05 g·L^(-1),蛋白质含量为49.06%.这表明,蛋白核小球藻CP-FJ9是一株有潜力用于开发富含蛋白质的藻种.Chlorella pyrenoidosa CP-FJ9 as the starting strain was cultured in SE, HA-SK,BG11,Basal, and BBM media respectively under heterotrophic conditions.The effects of five media on the growth and the product synthesis of C.pyrenoidosa CP-FJ9 were compared by measuring biomass, specific growth rate and main components in cells.The results showed that Basal medium was more suitable for the rapid cultivation of C.pyrenoidosa CP-FJ9,and the highest biomass(12.93 g·L^(-1)) and maximum specific growth rate(0.64 d^(-1)) were obtained;While HA-SK medium was more conductive to the protein synthesis of C.pyrenoidosa CP-FJ9, and the highest protein yield reached 5.14 g·L^(-1). In addition, this microalga heterotrophically cultivated with HA-SK medium in the 10 L fermenter attained, after 72 hours of fermentation, the final biomass of 20.05 g·L^(-1)with 49.06% protein content.Taken together, these results show that C.pyrenoidosa CP-FJ9 isa potential strain for the development of protein-rich algae.

关 键 词：蛋白核小球藻 异养培养 培养基 产物合成 

分 类 号：Q939.9[生物学—微生物学]