经眼蓝光无创光疗对PTSD模型大鼠焦虑恐惧负性情绪的影响  

Interventional Effect of Non-Invasive Transocular Phototherapy on Negative Emotions in Post-Traumatic Stress Disorder Model Rats

作　　者：李怡 谭一舟 王玮文[3,4] 戴佳婕 曾晶 顾瑛 LI Yi;TAN Yizhou;WANG Weiwen;DAI Jiajie;ZENG Jing;GUYing(Medical School of Chinese PLA,Beijing 100853,China;Department of LaserMedicine,TheFirst Medical Center of Chinese PLA General Hospital;CAS Key Laboratory of Mental Health,Institute of Psychology;Department of Psychology,University of Chinese Academy of Sciences;School of Medical Technology,Beijing Instituteof Technology)

机构地区：[1]解放军医学院,北京市100853 [2]中国人民解放军总医院第一医学中心激光医学科 [3]中国科学院心理研究所 [4]中国科学院大学心理学系 [5]北京理工大学医学技术学院

出　　处：《中国激光医学杂志》2022年第6期313-319,356,357,共9页Chinese Journal of Laser Medicine & Surgery

基　　金：海南省重大科技计划项目(ZDKJ2019012);国家自然基金资助(61835009,82071517);中国科学院心理研究所资助(E2CX4115CX);中国医学科学院医学与健康科技创新工程(2019-I2M-5-061)。

摘　　要：目的研究窄带蓝光经眼无创光疗对创伤后应激障碍(post-traumatic stress disorder,PTSD)模型大鼠的干预作用,以及光疗对基底外侧杏仁核(basolateral amygdala,BLA)区神经元激活水平的影响。方法实验对象SD大鼠24只,随机分为空白对照组、应激对照组与光疗组三组,每组各8只大鼠。其中空白对照组大鼠正常饲养,不进行建模和光疗处理;应激对照组采用单次延长应激结合足底电击模型(single inescapable electric foot shock after single prolonged stress procedure,SPS-S)应激处理后,建立PTSD模型但不进行无创光疗干预;光疗组采用SPS-S法应激处理后建立PTSD模型,于应激处理后10 d开始,每天上午8~9点经眼进行波长470 nm窄带LED无创光疗干预,连续照射14 d,照度500 lux。分别于应激处理后第7天、无创光疗处理后第14天,利用旷场实验(open field test,OFT)、高架十字迷宫(elevated plus maze,EPM)检测大鼠的焦虑症状;使用木僵实验检测大鼠的恐惧症状,评估PTSD模型构建和效度、经眼无创光疗逆转应激诱导的焦虑行为情况。三组采用相应处理后,在行为学检测后1~1.5 h内,各取6只大鼠进行灌注和免疫荧光染色,观察c-fos表达水平以评估情绪调节重要脑区基底外侧杏仁核(basolateral amygdala,BLA)区的神经元激活水平。结果(1)SPS-S法应激处理后第7天,旷场实验结果为应激对照组、光疗组大鼠进入中央区的次数、中央区停留时间均显著低于空白对照组(均P<0.001);高架十字迷宫实验结果为应激对照组、光疗组大鼠进入开放臂的次数、开放臂停留时间比均显著低于空白对照组(均P<0.001);木僵实验结果为应激对照组、光疗组大鼠与空白对照组比较,木僵时间百分比显著增加(均P<0.05),成功构建PTSD动物模型。(2)经眼无创光疗处理后第14天,旷场实验结果为光疗组大鼠进入旷场中央区的次数、中央区停留时间均显著多于应激对照组(均P<0.05),�Objective To study the intervention effect of non-invasive transocular phototherapy with narrow-band blue light on post-traumatic stress disorder(PTSD)model rats and the potential brain regions of phototherapy intervention.Methods Totally 24 SD rats were randomly allocated into a control group,stress group and light group.Single Inescapable Electric Foot Shock after Single Prolonged Stress Procedure(SPS-S for short)was used to construct PTSD model rats.470 nm narrow-band blue light of the illuminance of 500 lux was used to perform non-invasive phototherapy intervention on SPS-S rats from 8:00 to 9:00 am each day in 2 weeks.The rats in the control group received neither SPS-S nor phototherapy.The rats in the stress group were given SPS-S but no phototherapy.The rats in the light group were given both SPS-S and phototherapy.Open field test and elevated plus maze test were used to determine the anxiety level of the rats.Freezing was used to evaluate their fear levels.6 rats of the 3 groups sacrificed 1-1.5 h after the freezing test and paraformaldehyde perfusion was applied for the preservation of brain samples.Immunofluorescence staining with c-fos was used to evaluate the changes in the activation level of neurons in the basolateral amygdala(BLA).Results(1)Seven days after SPS-S:In the open field test,the number of entries(P<0.001)and dwell time(P<0.001)in the central area decreased more in the stress and phototherapy groups than in the control group.In elevated plus maze test,the number of entries(P<0.001)and the percentage of dwell time(P<0.001)in the open arm decreased more in the stress and phototherapy groups than in the control group.In freezing test,the percentage of freezing time(P<0.05)increased in the stress and phototherapy groups.(2)Fourteen days after phototherapy:In the open field test,the number of entries(P<0.05)and dwell time(P<0.05)in the central area of the open field were much more in the phototherapy and control group than in the stress group.The results of elevated plus maze showed that the n

关 键 词：无创光疗 创伤后应激障碍 SPS-S 

分 类 号：R318.51[医药卫生—生物医学工程] B845.1[医药卫生—基础医学]