Regulation effect of osteoblasts towards osteocytes by silk fibroin encapsulation  

作　　者：Dandan LUO Rui ZHANG Shibo WANG M.Zubair IQBAL Ruibo ZHAO Xiangdong KONG 

机构地区：[1]Institute of Smart Biomedical Materials,School of Materials Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China [2]Zhejiang-Mauritius Joint Research Centerfor Biomaterials and Tissue Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China [3]School of Textile Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China

出　　处：《Frontiers of Materials Science》2022年第4期1-13,共13页材料学前沿（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.51902289 and 51672250);the Natural Science Foundation of Zhejiang Province(Grant No.LQ19E020010);the Project of Zhejiang Provincial Department of Education(Grant No.Y201840323);the Key Project of Zhejiang Science and Technology(Grant Nos.2021C01180 and 2019C04020);the Research Foundation of ZSTU(Grant Nos.18012134-Y and 2020Q008).

摘　　要：Herein,the rational design micromilieus involved silk fibroin(SF)-based materials have been used to encapsulate the osteoblasts,forming an extracellular coated shell on the cells,which exhibited the high potential to shift the regulation of osteoblasts to osteocytes by encapsulation cues.SF coating treated cells showed a change in cell morphology from osteoblasts-like to osteocytes-like shape comparedwith untreated ones.Moreover,the expression of alkaline phosphatase(ALP),collagen I(Col I)and osteocalcin(OcN)further indicated a potential approach for inducingosteoblasts regulation,which typically accelerates calcium deposition and cell calcification,presenting a key role for the SF encapsulation in controlling osteoblasts behavior.This discovery showed that SF-based cell encapsulation could be used for osteoblasts behavior regulation,which offers a great potential to modulate mammalian cells'phenotype involvingalternatingsurrounding cues.

关 键 词：Cell encapsulation silk fibroin osteoblasts modulation cell differentiation cell calcification 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]