干旱胁迫下玉米苗期的转录组分析  

Transcriptome Analysis of Maize Seedlings under Drought Stress

作　　者：胡玉锋 金峰学[2] 程云清[1] 单晓辉[4] 李毅丹[2,3] 姜志磊 郝文媛[2] HU Yufeng;JIN Fengxue;CHENG Yunqing;SHAN Xiaohui;LI Yidan;JIANG Zhilei;HAO Wenyuan(School of Life Science,Jilin Normal University,Siping 136000;Jilin Academy of Agricultural Sciences,Changchun 130033;Jilin Provincial Key Laboratory of Agricultural Biotechnology,Changchun 130033;College of Plant Science,Jilin University,Changchun 130062,China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林省农业科学院,长春130033 [3]吉林省农业生物技术重点实验室,长春130033 [4]吉林大学植物科学学院,长春130062

出　　处：《东北农业科学》2022年第6期48-52,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程(CXGC2018ZY025);吉林省国际科技合作项目(20200801070GH);吉林省已完成绩效评估创新中心科研项目(20200602049ZP)。

摘　　要：干旱是影响玉米产量的重要因素。为了探究玉米的抗旱机制,本研究以干旱胁迫前后的B73玉米为实验材料,取地上部分采用IlluminaHiSeq 2000平台对其进行转录组测序。对测序数据进行基因差异表达分析,共鉴定出差异表达基因1111个,其中上调表达基因963个,下调表达基因148个。对差异表达基因进行了GO和KEGG功能富集分析,结果表明在干旱胁迫下差异基因主要富集在细胞壁合成、糖基化过程相关的代谢途径中,说明这两个代谢途径与玉米抗旱性密切相关。Drought is an important factor affecting maize yield.In order to explore the drought resistance mechanism of maize seedlings,this study used IlluminaHiseq 2000 to sequence the transcriptome of B73 maize seedlings under the well watering and drought stress conditions.The filtered data was screened for gene differential expression,and the screened differentially expressed genes were analyzed by GO and KEGG.The results showed that differential genes were mainly enriched in cell wall synthesis and glycosylation pathways under the drought stress,which indicated that these two pathways might be closely related to drought resistance in maize seedlings.

关 键 词：玉米 转录组 干旱 细胞壁 糖基转移酶 

分 类 号：S513[农业科学—作物学]