Toll样受体7、CTLA-4基因多态性与重症哮喘的关联性分析  

Association of Toll-like receptor 7,CTLA-4 gene polymorphisms with severe asthma

作　　者：罗坤[1] 徐淑凤[1] 赵靖[1] 刘菲菲[1] 王晓杰[1] LUO Kun;XU Shufeng;ZHAO Jing;LIU Feifei;WANG Xiaojie(Respiratory Department,Qinhuangdao First Hospital,Qinhuangdao,Hebei 066000,China)

机构地区：[1]河北省秦皇岛市第一医院呼吸科,河北秦皇岛066000

出　　处：《公共卫生与预防医学》2023年第2期122-126,共5页Journal of Public Health and Preventive Medicine

基　　金：河北省重点研发计划项目(182777110D);秦皇岛市重点研发项目(201703A135)。

摘　　要：目的探讨Toll样受体7、CTLA-4基因多态性与重症哮喘的关联性。方法选取2018年2月至2020年3月秦皇岛市第一医院呼吸科收治的175例哮喘患者作为研究对象(轻症109例、重症66例),同期纳入该院门诊健康体检者248例为正常对照组。测定上述各组Toll样受体7、CTLA-4基因多态性,通过计算比值比(OR)和95%置信区间(CI)评估Toll样受体7、CTLA-4多态性与重症哮喘之间的关系,应用logistic回归分析评估Toll样受体7、CTLA-4多态性位点的基因型与重症哮喘的关联强度。结果重症哮喘组、轻症哮喘组TLR7 rs3853839 CC基因型比例、CTLA-4 rs231725 AA基因型比例、TLR7 rs3853839 C等位基因频率、CTLA-4 rs231725 A等位基因频率高于正常对照组(P<0.05),重症哮喘组TLR7 rs3853839 CC基因型比例、CTLA-4 rs231725 AA基因型比例、TLR7 rs3853839 C等位基因频率、CTLA-4 rs231725 A等位基因频率高于轻症哮喘组(P<0.05)。TLR7 rs3853839 CC基因型(OR=10.32、95%CI:5.59~23.89)、CTLA-4 rs231725 AA基因型(OR=13.21、95%CI:3.58-20.25)、TLR7 rs3853839 C等位基因频率(OR=11.328、95%CI:4.25~21.14)、CTLA-4 rs231725 A等位基因频率(OR:13.24、95%CI:6.59~20.21)均能增加重症哮喘易感性(P<0.05)。TLR7 rs3853839CC基因型、TLR7 rs3853839C等位基因频率、CTLA-4 rs231725AA基因型、CTLA-4 rs231725A等位基因频率均为重症哮喘发生的危险因素(P<0.05)。结论TLR7 rs3853839 CC基因型、TLR7 rs3853839 C等位基因频率、CTLA-4 rs231725 AA基因型、CTLA-4 rs231725 A等位基因频率与重症哮喘发生存在一定关联性。Objective To explore the association of Toll-like receptor 7,CTLA-4 gene polymorphisms and severe asthma.Methods From February 2018 to March 2020,175 asthma patients admitted to the respiratory department of our hospital were selected as the research subjects(109 cases of mild disease and 66 cases of severe disease),and 248 cases of healthy people who were included in the outpatient physical examination of our hospital during the same period were selected as the normal control group.Toll-like receptor 7 and CTLA-4 gene polymorphisms in the above groups were determined,and the relationship between Toll-like receptor 7 and CTLA-4 polymorphisms and severe asthma was evaluated by calculating the odds ratio(OR)and 95%confidence interval(CI).The relationship between the genotypes of Toll-like receptor 7 and CTLA-4 polymorphisms and severe asthma were evaluated by logistic regression analysis.Results The proportion of TLR7 rs3853839 CC genotype,CTLA-4 rs231725 AA genotype,TLR7 rs3853839 C allele frequency and CTLA-4 rs231725 A allele frequency in severe asthma group and mild asthma group were higher than those in normal control group(P<0.05).The proportion of TLR7 rs3853839 CC genotype,the proportion of CTLA-4 rs231725 AA genotype,the frequency of TLR7 rs3853839 C allele,and the frequency of CTLA-4 rs231725 A allele in the severe asthma group were higher than those in the mild asthma group(P<0.05).TLR7 rs3853839 CC genotype(OR=10.32,95%CI=5.59-23.89),CTLA-4 rs231725 AA genotype(OR=13.21,95%CI=3.58-20.25),TLR7 rs3853839 C allele frequency(OR=11.32,95%CI=4.25-21.14)and CTLA-4 rs231725 A allele frequency(OR=13.24,95%CI=6.59-20.21)could increase the susceptibility to severe asthma(P<0.05).TLR7 rs3853839CC genotype,TLR7 rs3853839C allele frequency,CTLA-4 rs231725AA genotype and CTLA-4 rs231725A allele frequency were risk factors for severe asthma(P<0.05).Conclusion TLR7 rs3853839 CC genotype,TLR7 rs3853839 C allele frequency,CTLA-4 rs231725 AA genotype and CTLA-4 rs231725 A allele frequency are associated with the occurrence

关 键 词：TOLL样受体7 CTLA-4 基因多态性 重症哮喘 

分 类 号：R181[医药卫生—流行病学]