基于氧化石墨烯的适配体生物传感器检测Hg^(2+)  

GO-based aptamer biosensor for Hg^(2+)detection

作　　者：李云霞[1] 马莉萍[1] 马生龙[1] 聂莹莹[1] LI Yunxia;MA Liping;MA Shenglong;NIE Yingying(Key Laboratory of Sensor and Sensing Technology of Gansu Province,Institute of Sensor Technology,Gansu Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]甘肃省科学院传感技术研究所甘肃省传感器与传感技术重点实验室,甘肃兰州730000

出　　处：《传感器与微系统》2023年第3期31-35,共5页Transducer and Microsystem Technologies

基　　金：兰州市城关区科技计划资助项目(2020—2—1—1)。

摘　　要：针对汞离子(Hg^(2+))作为一种毒性强且污染较为普遍的重金属离子,会严重危害人体健康的问题,设计了Hg^(2+)特异识别探针T_(28)-FAM,T_(28)-FAM可与Hg^(2+)共价结合形成稳定的T-Hg^(2+)-T配位结构,以T_(28)-FAM为荧光信号源,氧化石墨烯(GO)为猝灭剂,构建基于T_(28)-FAM/GO的适配体荧光传感器,实现对Hg^(2+)浓度的高灵敏定量检测。该传感器检测Hg^(2+)的线性范围为100~600 nmol/L,相关系数R^(2)为0.996 12,检出限为0.458 nmol/L。9种干扰离子(Co^(2+),Cu^(2+),Cl^(-),Mg^(2+),Ca^(2+),Ag^(+),Fe^(2+),Pb^(2+),NO_(3)^(-))的存在不影响该方法检测Hg^(2+),表明该方法对Hg^(2+)检测具有较好的特异性。在自来水样Hg^(2+)加标检测实验中,平均回收率为95.77%,有望用于实际样品的检测。Aiming at the problem that mercury is a heavy metal ion with strong toxicity and common pollution, which can cause serious harm to human health.Hg^(2+)-specific recognition probe T_(28)-FAM is designed, which can covalently combine with Hg^(2+)to form a stable T-Hg^(2+)-T coordination structure. Using T_(28)-FAM as the fluorescence signal source and graphene oxide(GO) as quencher, an aptamer fluorescence sensor based on T_(28)-FAM/GO is constructed to realize the highly sensitive quantitative detection on Hg^(2+)concentration. The linear range of the sensor for Hg^(2+)detection is 100~600 nmol/L,the correlation coefficient R^(2) is 0.996 12,the detection limit is 0.458 nmol/L. The presence of nine interfering ions(Co^(2+),Cu^(2+),Cl^(-),Mg^(2+),Ca^(2+),Ag+,Fe^(2+),Pb^(2+),NO_(3)^(-)) do not affect on detection of Hg^(2+)by this method, which indicates that this method has good specificity for Hg^(2+)detection. The average recovery of Hg^(2+)in water samples is 95.77 %, which has a good application prospect in the detection of actual samples.

关 键 词：氧化石墨烯 适配体生物传感器 汞离子 荧光检测 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O657.1[自动化与计算机技术—控制科学与工程]