芝麻素通过PI3K/AKT途径对宫颈癌细胞增殖、凋亡和迁移的影响  

Effects of sesamin on the proliferation,apoptosis and migration of cervical cancer cells through PI3K/AKT pathway in vitro

作　　者：方娟 张远远 王燕[2] 肖波[1] FANG Juan;ZHANG Yuanyuan;WANG Yan(Department of Obstetrics and Gynecology,Hanyang Hospital Affiliated to Wuhan University of Science and Technology,Hubei,Wuhan 430050,China;不详)

机构地区：[1]武汉科技大学附属汉阳医院妇产科,武汉市430050 [2]湖北省妇幼保健院妇产科

出　　处：《河北医药》2023年第1期26-29,34,共5页Hebei Medical Journal

基　　金：湖北省自然科学基金(编号:WJ2017S006)。

摘　　要：目的探讨芝麻素通过调控磷脂酰肌醇3激酶/蛋白激酶B(PI3K/AKT)途径对宫颈癌细胞增殖、凋亡和迁移的影响。方法体外培养人宫颈癌细胞株Hela,随机分为对照组、芝麻素低剂量(20μg/ml)组、芝麻素中剂量(40μg/ml)组、芝麻素高剂量(80μg/ml)组。CCK-8法检测芝麻素对Hela细胞增殖的影响;Transwell实验检测芝麻素对Hela细胞迁移能力的影响;流式细胞术检测细胞凋亡情况;蛋白质印迹法检测增殖细胞核抗原(PCNA)、基质金属蛋白酶-9(MMP-9)、B淋巴细胞瘤-2基因(Bcl-2)和与其同源的促凋亡蛋白Bax、半胱氨酸蛋白酶(Caspase)-3、Caspase-9、PI3K、p-PI3K、Akt和p-Akt蛋白表达情况。结果与对照组比较,芝麻素低、中、高剂量组OD490值、迁移细胞数、PCNA、MMP-9、Bcl-2、p-PI3K和p-Akt蛋白表达水平依次降低(P<0.05),细胞凋亡率、Bax、Caspase-3和Caspase-9蛋白表达水平依次升高,且呈现剂量依赖性(均P<0.05)。结论芝麻素可抑制宫颈癌细胞增殖、迁移并促进凋亡,其机制可能与抑制PI3K/AKT途径有关。Objective To investigate the Effects of sesamin on the proliferation, apoptosis and migration of cervical cancer cells by regulating the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT) pathway in vitro.Methods The human cervical cancer cell line Hela was cultured in vitro and randomly divided into control group, low-dose, medium-dose, high-dose sesamin groups(20μg/ml, 40μg/ml, 80μg/ml, respectively).CCK-8 method was used to detect the effects of sesamin on the proliferation of Hela cells;Transwell experiment was used to detect the effects of sesamin on the migration ability of Hela cells;flow cytometry was used to detect cell apoptosis;Western Blot was used to detect the protein expression levels of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase-9(MMP-9),B cell lymphoma-2 gene(Bcl-2) and its homologous pro-apoptotic protein-Bax and cysteine protease(Caspase)-3,Caspase-9,PI3K,p-PI3K,Akt and p-Akt.Results Compared with those in control group, the OD490 value, the number of migrating cells, and the expression levels of PCNA,MMP-9,Bcl-2,p-PI3K and p-Akt protein in low-dose, medium-dose and high-dose sesamin groups were significantly decreased in order(P<0.05),however, the apoptotic rate, and the expression levels of Bax, Caspase-3 and Caspase-9 protein were significantly increased in order, with a dose-dependent manner(P<0.05).Conclusion Sesamin can inhibit the proliferation and migration of cervical cancer cells and promote apoptosis, and its action mechanism may be related to the inhibition of PI3K/AKT pathway.

关 键 词：芝麻素 磷脂酰肌醇3激酶/蛋白激酶B途径 宫颈癌 增殖 凋亡 迁移 

分 类 号：R711.74[医药卫生—妇产科学]