萎缩芽胞杆菌(Bacillus atrophaeus)GJW2-1可湿性粉剂研制及对党参灰霉病的防治效果  

Preparation of Wettable Powder of Bacillus atrophaeus GJW2-1 and Its Control Effect on Gray Mold of Dangshen(Codonopsis pilosula)

作　　者：陈大为 周天琦 张培怡 庹顺羽 贾凌云[1] 候勤正 孙坤[1] CHEN Da-wei;ZHOU Tian-qi;ZHANG Pei-yi;TUO Shun-yu;JIA Ling-yun;HOU Qin-zheng;SUN Kun(Coll.of Life Sci.,NW Normal Uni.,Lanzhou 730070)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《微生物学杂志》2022年第6期87-93,共7页Journal of Microbiology

基　　金：甘肃省重点研发计划项目(农业类)(18YF1NA051)。

摘　　要：以萎缩芽胞杆菌(Bacillus atrophaeus)GJW2-1为试验材料,研制一种对党参灰霉病具有良好防治效果的可湿性粉剂。采用单因素法和正交试验法筛选出对菌株GJW2-1活性影响最小的载体和助剂及其配比,并对其稳定性和盆栽防效进行评价。结果表明,萎缩芽胞杆菌GJW2-1可湿性粉剂最佳配方为载体高岭土35%,分散剂羧甲基纤维素钠4%,紫外保护剂β-环糊精3%,湿润剂十二烷基苯磺酸钠10%。该可湿性粉剂活菌含量1×10^(9)cfu/g,湿润时间57 s,悬浮率81%,pH值7.1,含水率2.03%,且具有较好的稳定性。盆栽防效结果表明,该可湿性粉剂100倍稀释液对党参灰霉病的预防和治疗效果最好,分别为81.75%和71.56%。Biocontrol agent of wettable powder with good efficacy on gray mold of Codonopsis pilosula was studied,Bacillus atrophus GJW2-1 was taken as test material to screen the carriers and auxiliaries with the least influence on the activity of strain GJW2-1 by single factor and orthogonal test,and its stability and potted control effect were evaluated.The results showed that the optimum formula of the wettable powder included 35%kaolin,4%carboxymethyl cellulose sodium as dispersant,3%β-cyclodextrin as UV protector,10%sodium dodecyl benzene sulfonate as humectant.The content of viable bacteria was 1×10^(9) cfu/g in the wettable powder,its wetting time was 57 s,suspension rate was 81%,pH 7.1 and rate of water content was 2.03%,and had good stability.The results of potted control effect showed that the wettable powder 100 times diluent had the highest prevention and treatment effect on gray mold of Codonopsis pilosula,which were 81.75%and 71.56%,respectively.

关 键 词：萎缩芽胞杆菌 正交优化 可湿性粉剂 党参灰霉病 防治效果 

分 类 号：Q939.96[生物学—微生物学]