基于SSR分子标记技术的黄淮海玉米杂优模式分析  

Analysis of Huang-Huai-Hai maize heterotic pattern based on SSR molecular marker technology

作　　者：柴文波 李淑芬 李洪涛[1] 许瀚元[1] 祝庆 王军[1] CHAI Wenbo;LI Shufen;LI Hongtao;XU Hanyuan;ZHU Qing;WANG Jun(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,China)

机构地区：[1]连云港市农业科学院,江苏连云港222000

出　　处：《北方农业学报》2022年第6期17-24,共8页Journal of Northern Agriculture

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS〔2021〕055);江苏省连云港市财政专项(QNJJ2202,QNJJ2110,QNJJ2003,QNJJ2106);江苏省农业重大新品种创制项目(PZCZ201711);苏北科技专项(SZ-LYG202141)。

摘　　要：【目的】分析玉米优异育种材料L239和L7221的杂优模式,揭示优异品种的基因来源,为黄淮海地区的玉米选育提供参考。【方法】采用SSR分子标记技术对L239和L7221在42份玉米自交系中的遗传多样性进行分析,确定黄淮海地区的玉米杂优模式。【结果】对42份玉米自交系进行分析发现,56条引物的等位基因数为2~9,多态性信息量为0.43~0.86。L239与CA375的遗传距离最近,为0.50;L7221与LX9801和农大178的遗传距离较近,分别为0.43和0.56。对42份玉米自交系的遗传相似性分析可知,L7221主要属于塘四平头群,L239主要与改良Reid群的种质资源关系比较近。【结论】L7221与塘四平头群多个自交系亲缘关系较近,L239与改良Reid群多个自交系亲缘关系较近;L239和L7221测配的杂优模式为改良Reid群×塘四平头群。【Objective】Analyzing the heterotic pattern of superior maize breeding lines L239 and L7221 to reveal the genetic resources of superior varieties and to provide reference for maize breeding in the Huang-Huai-Hai region.【Methods】The genetic diversity of L239 and L7221 in 42 maize inbred lines was analyzed by SSR molecular marker technology to determine the heterosis pattern of maize in Huang-Huai-Hai region.【Results】Analysis of 42 maize inbred lines showed that the allele number of the 56 primers was 2-9,and the polymorphism information content was 0.43-0.86.The genetic distance between L239 and CA375 was shortest at 0.50.The genetic distance between L7221,LX9801 and Nongda 178 were shorter at 0.43 and 0.56,respectively.According to the genetic similarity analysis of 42 maize inbred lines,L7221 mainly belonged to Tangsipingtou group,and L239 mainly had closer relationship with the germplasm resources of the improved Reid group.【Conclusion】L7221 was closer to many inbred lines of Tangsipingtou group,and L239 was closer to many inbred lines of improved Reid group.The L239 and L7221 heterosis pattern was improved Reid group×Tangsipingtou group.

关 键 词：玉米 L239 L7221 杂优模式 SSR 

分 类 号：S513[农业科学—作物学]