鸡树条果多糖的抗氧化活性研究  

Study on Anti-oxidative Activity of Polysaccharide from Fruit of Sargent Viburnum

作　　者：高长久[1] 梁启超[1] 王春辉 张朝立[1] 孟令锴[1] 吴兆华[1] 吴宜艳[1] 王晶华[1] 李文超[1] GAO Changjiu;LIANG Qichao;WANG Chunhui;ZHANG Chaoli;MENG Lingkai;WU Zhaohua;WU Yiyan;WANG Jinghua;LI Wenchao(College of Pharmacy,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院药学院,黑龙江牡丹江157011

出　　处：《中国中医药现代远程教育》2023年第7期163-165,共3页Chinese Medicine Modern Distance Education of China

基　　金：黑龙江省教育厅省属高等学校基本科研业务费科研项目【No.2020-KYYWFMY-0050】;黑龙江省卫生健康委科研课题【No.2020-420】;牡丹江市科学技术局应用技术研究与开发计划项目【No.HT2020NS093】。

摘　　要：目的通过研究鸡树条果多糖的抗氧化活性,为其开发利用提供科学依据。方法以维生素C为阳性对照,分别采用DPPH法、邻苯三酚自氧化法和邻二氮菲-金属铁离子-H_(2)O_(2)法测定0.2~1.2 mg/mL浓度范围内鸡树条果多糖对DPPH·、·OH和O_(2)^(-)·的清除活性。结果在一定浓度范围内,鸡树条果多糖对DPPH·、·OH和O_(2)^(-)·3种自由基均具有清除作用,且随浓度增加,其清除能力随之增强。当质量浓度达到1.2 mg/mL时,清除率分别达到82.8%、76.7%和73.2%。结论鸡树条果多糖对DPPH·、·OH和O_(2)^(-)·3种自由基均有清除作用,是天然有效的抗氧化剂。Objective To provide a scientific basis for its development and utilization by studying the antioxidant activity of polysaccharide from fruit of sargent viburnum.Methods With Vc as the positive control,DPPH method,pyrogallol auto-oxidation method and phenanthroline-metal iron ion-H_(2)O_(2)method were used to determine the effect of polysaccharide from Fruit of Sargent Viburnum on DPPH in the concentration range of 0.2~1.2mg/ml.,OH and O_(2)-scavenging activities.Results Within a certain concentration range,the polysaccharide from Fruit of Sargent Viburnum had scavenging effects on three free radicals,DPPH,OH and O_(2)^(-),and its scavenging ability increased with the increase of concentration.When the mass concentration reached 1.2 mg/ml,the clearance rates reached 82.8%,76.7%and 73.2%,respectively.Conclusion The polysaccharide from fruit of sargent viburnum has scavenging effects on three free radicals of DPPH,OH and O_(2)^(-),and is a natural and effective antioxidant.

关 键 词：鸡树条果 多糖 抗氧化 实验研究 

分 类 号：R284.1[医药卫生—中药学]