CD30/CD30L通路调控实验性脑疟发生机制的研究  

Mechanism through which the CD30/CD30L pathway mediates experimental cerebral malaria

作　　者：刘文婷 姚世杰 赵艳 卞致芳 温易鑫 崔泽实[3] 曹雅明 LIU Wen-ting;YAO Shi-jie;ZHAO Yan;BIAN Zhi-fang;WEN Yi-xin;CUI Ze-shi;CAO Ya-ming(Department of Immunology,Basic Medicine College,China Medical University,Shenyang 110122,China;The Third Cadre Medical Ward,Affiliated Central Hospital of Shenyang Medical College,Shengyang 110020,China;School of Pharmacy,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110122 [2]沈阳医学院附属中心医院第三干诊病房,沈阳110020 [3]中国医科大学药学院,沈阳110122

出　　处：《中国人兽共患病学报》2023年第2期131-134,179,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81871683)。

摘　　要：目的探讨CD30/CD30L信号通路在实验性脑疟发生过程中的作用及机制。方法以1×10^(6)个伯氏疟原虫(Plasmodium berghhei ANKA,PbA)寄生的红细胞腹腔感染C57BL/6及C57BL/6背景的CD30L基因敲除鼠,动态检测红细胞感染率,同时观察实验性脑疟发生情况并统计其生存率。流式细胞术检测感染后3 d和5 d脾细胞中Th1和MDSC百分率,DC中cDC和pDC的百分率以及表达PD-L1细胞的平均荧光强度,CD_(4)^(+)T细胞中Treg和表达PD-1细胞的百分率。结果与对照组相比,CD30L敲除鼠红细胞感染率未出现显著性变化,但具有更高的脑疟发生率和死亡率;pDC、Th1细胞的百分率于感染后3 d或5 d出现显著升高,而MDSC和Treg以及表达PD-1细胞的百分率和表达PD-L1细胞的平均荧光强度明显下降。结论CD30/CD30L通路通过下调pDC和上调MDSC、Treg以及表达PD-1和PD-L1细胞比率而拮抗Th1应答并以此调控实验性脑疟的发生。To explore the role and mechanism of CD30/CD30L signaling pathway in the pathogenesis of cerebral malaria,we intra-peritoneally infected C57BL/6 mice and CD30L knockout mice on a C57BL/6 background with erythrocytes parasitized with Plasmodium berghei ANKA.Parasitemia,survival and the incidence rate of experimental cerebral malaria were dynamically detected.Flow cytometry was used to detect the percentages of Th1 and MDSCs in spleen cells,cDCs and pDCs in DCs,the average fluorescence intensity of PD-L1 expressing cells,and the percentages of Treg and PD-1 expressing cells among CD_(4)^(+)T cells at 3 and 5 days after infection.Compared with that in the control group,the infection rate of red blood cells in CD30L knockout mice did not significantly differ,but the incidence and mortality of cerebral malaria were higher.The percentages of pDC and Th1 cells increased significantly 3 or 5 days after infection,whereas the percentages of MDSC,Treg and pD-1-expressing cells,and the average fluorescence intensity of PD-L1 cells,decreased significantly.The CD30/CD30L pathway antagonizes the Th1 response by down-regulating pDCs,up-regulating MDSCs and Tregs,and expressing the PD-1 and PD-L1 cell ratio,thereby regulating the occurrence of experimental cerebral malaria.

关 键 词：CD30/CD30L通路 免疫调控 TH1应答 实验性脑疟 

分 类 号：R382.3[医药卫生—医学寄生虫学]