银椴苷对激活的原代小胶质细胞极化及神经炎症的影响  

The Effect of Tiliroside on the Polarization and Neuroinflammation of Activated Primary Microglia

作　　者：申小年[1] 王俐君 王星禹[3] 张宗奇[4] 徐颖[3] Shen Xiaonian;Wang Lijun;Wang Xingyu;Zhang Zongqi;Xu Ying(Department of Medical Care,Anhui College of Traditional Chinese Medicine,Wuhu 241000,China;School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Physiology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200021,China)

机构地区：[1]安徽中医药高等专科学校医疗系,芜湖241000 [2]上海中医药大学康复医学院,上海201203 [3]上海中医药大学生理教研室,上海201203 [4]上海中医药大学附属曙光医院,上海200021

出　　处：《世界科学技术-中医药现代化》2022年第10期3806-3812,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目(82174003):异甘草素通过小胶质细胞调节vCA1区PV阳性中间神经元兴奋性改善AD早期突触功能紊乱的机制研究,负责人:徐颖;安徽省级教育厅重点项目(KJ2019A1093):应用膜片钳技术评价定痫丸对KCNQ2/3离子通道的药理作用及有效成份筛选,负责人:申小年。

摘　　要：目的银椴苷(Tiliroside,Tle)是一种天然产物,存在于结香花(Edgeworthia chrysantha Lindl.)、金英(Galphimiagracilis)、和Phlomoidesspectabilis等生物体中。本文主要探讨Tle对脂多糖(lipopolysaccharide,LPS)激活的原代小胶质细胞的M1/M2极化的转化作用及其抑制神经炎症反应的影响。方法MTT检测各浓度Tle对LPS激活的原代小胶质细胞活性的影响;Griess试剂检测LPS激活的原代小胶质细胞的一氧化氮(Nitric oxide,NO)产物生成量的影响;ELISA法检测Tle对LPS激活的原代小胶质细胞的肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)蛋白水平的影响;qRT-PCR检测Tle对LPS激活的原代小胶质细胞的精氨酸酶-1(Arginase-1,Arg-1)和胰岛素生长因子-1(Insulin growth factor-1,IGF-1)的mRNA表达水平。结果Tle(0-80μmol·L^(-1))对LPS激活的原代小胶质细胞活性没有明显影响;Tle显著降低LPS激活的原代小胶质细胞亚硝酸盐的含量,并对炎症因子IL-6、TNF-α蛋白水平有显著抑制作用,同时显著提高抗炎因子Arg-1和IGF-1的mRNA水平。结论Tle能够通过促进LPS激活的原代小胶质细胞M1型向M2型转化从而抑制神经炎症反应,因此Tle具有一定的神经保护作用。Objective Tiliroside(TLE)is a natural product,which exists in Edgeworthia chrysantha Lindl.,galphimia gracilis,phlomoides spectabilis and other organisms.This paper mainly discusses the transformation of M1/M2 polarization of primary microglia activated by lipopolysaccharide(LPS)and its effect on inhibiting neuro inflammation.Methods MTT was used to detect the effect of each concentration of Tle on the activity of primary microglia activated by LPS;Griess reagent was used to detect the effect of nitric oxide(NO)product production in primary microglia activated by LPS;ELISA method to detect the effect of Tle on the protein levels of Tumor necrosis factor-α(TNF-α)and Interleukin-6(IL-6)in primary microglia activated by LPS;qRT-PCR detection of Tle on the mRNA expression of arginase-1(Arg-1)and insulin growth factor-1(IGF-1)in primary microglia activated by LPS Level.Results Tle(0-80μmol·L^(-1))had no significant effect on the activity of primary microglia activated by LPS;Tle significantly reduced the content of nitrite in primary microglia activated by LPS,and had an effect on the inflammatory factors IL-6 and TNF-αprotein level has a significant inhibitory effect,while significantly increasing the mRNA levels of anti-inflammatory factors Arg-1 and IGF-1.Conclusion Tle can inhibit the neuro-inflammatory response by promoting the transformation of primary microglia activated by LPS from M1 type to M2 type.Therefore,Tle has a certain neuroprotective effect.

关 键 词：银椴苷 小胶质细胞 M1/M2极化 神经炎症 

分 类 号：R285.5[医药卫生—中药学]