Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley,Malaysia  

作　　者：Nurul Diana Dzaraly Mohd Nasir Mohd Desa AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Nurul Hana Zainal Baharin Nurshahira Sulaiman Nurul Asyikin Abdul Rahman Navindra Kumari Palanisamy Zarizal Suhaili Semiat Opeyemi Yusuff Nor Iza A.Rahman Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah 

机构地区：[1]Department of Biomedical Sciences,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,UPM Serdang,Selangor,Malaysia [2]Department of Medical Microbiology,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,UPM Serdang,Selangor,Malaysia [3]School of Biology,Faculty of Applied Sciences,Universiti Teknologi MARA Negeri Sembilan,Kuala Pilah Branch,Kuala Pilah,Negeri Sembilan,Malaysia [4]Department of Medical Microbiology and Parasitology,Faculty of Medicine,Universiti Teknologi MARA,Sungai Buloh Campus,Jalan Hospital,Sungai Buloh,Selangor,Malaysia [5]School of Animal Science,Aquatic Science and Environment,Faculty of Bioresources and Food Industry,Universiti Sultan Zainal Abidin,Besut Campus,Besut,Terengganu,Malaysia [6]Faculty of Medicine,Universiti Sultan Zainal Abidin,Jalan Sultan Mahmud,Kuala Terengganu,Terengganu,Malaysia [7]Department of Pathology,Sungai Buloh Hospital,Jalan Hospital,Sungai Buloh,Selangor,Malaysia [8]Department of Pathology,Sultanah Nur Zahirah Hospital,Jalan Sultan Mahmud,Kuala Terengganu,Terengganu,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2023年第2期80-88,共9页亚太热带医药杂志(英文版)

基　　金：supported by Fundamental Research Grant Scheme(FRGS/1/2020/SKK0/UPM/02/10)of the Ministry of Higher Education Malaysia.

摘　　要：Objective:To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes,antibiotic resistance and genotype.Methods:In total,42 clinical isolates were collected between October 2017 and December 2019.Those isolates were analysed for antimicrobial susceptibility,serotype distribution,detection of pneumococcal virulence and pilus genes.Multilocus sequence typing was performed only for piliated isolates,followed by phylogenetic analysis.Results:The common isolation sites among the pneumococcal isolates were tracheal aspirate(28.6%),blood(26.2%),and sputum(23.8%).Fifty percent isolates were resistant to erythromycin,tetracycline(50.0%)and trimethoprim-sulfamethoxazole(43.0%).The most frequent were serotypes 19F(28.6%),6A/B(23.8%)and 19A(14.3%).Piliated isolates were detected in a small proportion(33.3%);64.3%were multidrug-resistant.ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14(CC271).In the phylogenetic analysis,some piliated isolates showed a close association having similar ST320,carrying serotype 19A and both pilus genes indicating their clonal spread.Conclusions:Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.

关 键 词：Pneumococci PILI GENOTYPE MALAYSIA 

分 类 号：R181.3[医药卫生—流行病学]