基于1,3-二氯-7-羟基-9,9-二甲基-2(9H)-吖啶酮(DDAO)的近红外荧光探针研究进展  

Research Progress of Near-Infrared Fluorescent Probes Based on 1,3-Dichloro-7-hydroxy-9,9-dimethyl-2(9H)-acridone(DDAO)

作　　者：马延慧 武宇乾 王晓旭 高贵 周欣 Ma Yanhui;Wu Yuqian;Wang Xiaoxu;Gao Gui;Zhou Xin(College of Science and Technology,Hebei Agricultural University,Cangzhou,Hebei 061100)

机构地区：[1]河北农业大学理工学院,河北沧州061100

出　　处：《有机化学》2023年第1期94-111,共18页Chinese Journal of Organic Chemistry

基　　金：2022年河北省在读研究生创新能力培养(No.CXZZSS2022101)资助项目。

摘　　要：1,3-二氯-7-羟基-9,9-二甲基-2(9H)-吖啶酮(DDAO)是一种优异的近红外荧光染料,具有近红外发射、低pK_(a)值(≈5.0)、高水溶性以及高量子产率(Φ=0.39),近年来在荧光探针领域受到广泛关注.研究证明,以该染料设计合成的荧光探针可以在温和的水溶液中工作,具有反应迅速、选择性和灵敏度高、检测限低以及比色信号变化明显等优异的传感性能.此外,该类探针的细胞毒性低,对于活细胞和生物体的检测具有显著的应用价值.综述了基于DDAO及其衍生物的荧光探针的研究进展,主要包括探针在生物酶、活性氧、活性氮、蛋白质以及Pd^(0)的识别检测中的分子设计、作用机理和应用,并对该类荧光探针的发展前景进行了展望.1,3-Dichloro-7-hydroxy-9,9-dimethyl-2(9H)-acridone(DDAO)is an excellent near-infrared fluorescent dye with near-infrared emission,low pK_(a)(≈5.0),high water solubility and high quantum yield(Φ=0.39),which has attracted much attention in recent years in the field of fluorescent probes.The researches prove that the fluorescent probes designed and synthesized based on this dye can work in mild aqueous solutions,and have excellent sensing performance,such as rapid reaction,high selectivity and sensitivity,low detection limit and obvious colorimetric signal change.In addition,the low cytotoxicity of this class of probes is of significant application for the detection of living cells and organisms.The research progress of fluorescent probes based on DDAO and its derivatives,mainly including the molecular design,action mechanism and application of probes in the recognition and detection of biological enzymes,reactive oxygen species,reactive nitrogen species,proteins and Pd^(0),is reviewed,and the development prospect of this type of fluorescent probes is prospected.

关 键 词：1 3-二氯-7-羟基-9 9-二甲基-2(9H)-吖啶酮(DDAO) 荧光探针 近红外 检测 

分 类 号：O657.3[理学—分析化学]