不同试剂处理对草莓植株生长及根际微生物群落结构的影响  

Effects of different reagent treatments on strawberry plant growth and rhizosphere microbial community structure

作　　者：朱丽 王庆莲[1,4] 唐山远 乔玉山 顾闽峰[3] 费月跃[3] 王伟义[3] 陈晓东[1] 赵密珍[1] ZHU Li;WANG Qing-lian;TANG Shan-yuan;QIAO Yu-shan;GU Min-feng;FEI Yue-yue;WANG Wei-yi;CHEN Xiao-dong;ZHAO Mi-zhen(Institute of Pomology,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Xinyang Experimental Station of Institute of Agricultural Sciences in Jiangsu Coastal Area,Yancheng 224049,China;White Rabbit Strawberry Research Institute of Jurong City in Jiangsu Province,Jurong 212400,China)

机构地区：[1]江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014 [2]南京农业大学园艺学院,江苏南京210095 [3]江苏沿海地区农业科学研究所新洋试验站,江苏盐城224049 [4]江苏省句容市白兔草莓研究院,江苏句容212400

出　　处：《江苏农业学报》2023年第1期198-207,共10页Jiangsu Journal of Agricultural Sciences

基　　金：江苏现代农业产业技术体系建设项目[JATS(2021)424];江苏省农业科技自主创新基金项目[CX(20)2021];江苏省重点研发计划项目(BE2018389)。

摘　　要：本研究以草莓宁玉为试材,以施加清水为对照(CK),分析宁盾微生物菌剂(JN)、哈茨木霉菌剂(JH)2种微生物菌剂和代森锰锌(YS)、口恶霉灵(YE)2种化学农药试剂对草莓植株生长及草莓根际微生物群落结构的影响。结果表明,JN处理的草莓植株在地上部生物量、根系生物量及总生物量等方面较CK、YS和YE处理组显著增加;在根系多酚氧化酶(PPO)活性和根系活力等生理生化特性上,JN处理也较CK和YE处理组显著提高。细菌16S rRNA和真菌内转录间隔区(ITS)对应基因测序结果显示,JN和JH处理的真菌Chao1指数和Shannon指数代表的根际基质真菌群落的丰富度和多样性指数较CK和YE处理显著减少。在门、属水平聚类分析中,JN和JH处理后的根际基质中变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)等有益菌门的菌群相对丰度较CK均有提高,芽孢杆菌属(Bacillus)等有益菌属的菌群相对丰度也高于CK和2种化学农药试剂处理。综上所述,与CK和2种化学农药试剂处理(YS、YE)比较,2种微生物菌剂处理(JN、JH)能有效促进草莓植株生长,其中宁盾微生物菌剂处理(JN)的表现最佳,并且2种微生物菌剂对草莓根际微生物群落结构也起到了一定的改善作用。In this study,strawberry Ningyu was used as the test material,and clear water was used as the control(CK)to analyze two microbial inoculants of Ningdun(JN)and Trichoderma harzianum(JH)and two chemical pesticide reagents of Mancozeb(YS)and Hymexazol(YE)on plant growth and microbial community structure in strawberry rhizosphere.The results showed that the aboveground biomass,root biomass and total biomass of strawberry plants under JN treatment increased significantly compared with CK,YS and YE treatment groups.The physiological and biochemical properties of root polyphenol oxidase(PPO)activity and root vigor were also significantly improved under JN treatment compared with CK and YE treatment groups.The results of bacterial 16S rRNA and fungal internal transcribed spacer(ITS)related gene sequencing showed that the richness and diversity indexes of the fungal community in the rhizosphere matrix represented by Chao1 index and Shannon index of the fungi under JN and JH treatments were significantly lower than those under CK and YE treatments.In the analysis of clustering at phylum and genus level,the relative abundance of beneficial bacteria such as Proteobacteria,Firmicutes and Actinobacteria in the rhizosphere matrix of JN and JH treatments increased compared with CK,and the relative abundance of beneficial bacterium such as Bacillus was also higher than that of CK and two chemical pesticide reagent treatments.In conclusion,compared with CK and two chemical pesticide reagent treatments(YS,YE),two microbial inoculant treatments(JN,JH)can effectively promote the growth of strawberry plants,and Ningdun microbial inoculant treatment(JN)shows the best performance.The two microbial inoculants also play a certain role in improving the microbial community structure of strawberry rhizosphere.

关 键 词：草莓 微生物菌剂 化学药剂 植株生长 根际微生物 

分 类 号：S668.4[农业科学—果树学]