SIRT3在早发型子痫前期胎盘组织中的表达及作用  

Expression and role of SIRT3 in placental tissue of patients with early-onset preeclampsia

作　　者：朱盛兰 都园渊 王少帅[1] 张慧婷 江一 张婧怡 周璇[1] 冯玲[1] Zhu Shenglan;Du Yuanyuan;Wang Shaoshuai(Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430000)

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430000

出　　处：《现代妇产科进展》2023年第2期102-107,112,共7页Progress in Obstetrics and Gynecology

基　　金：湖北省自然科学基金(No:2021cfb375)。

摘　　要：目的:检测早发型子痫前期(eoPE)患者胎盘组织中SIRT3表达,探讨SIRT3对滋养细胞功能的调控及其可能机制。方法:免疫组化法和免疫印迹法检测eoPE患者胎盘中SIRT3表达。试剂盒检测eoPE患者胎盘组织氧化应激因子水平。应用瞬时转染质粒使HTR-8/SVneo细胞过表达SIRT3,检测滋养细胞的侵袭和迁移能力。通过CoCl_(2)构建滋养细胞氧化应激模型,研究SIRT3对HTR-8/SVneo细胞抗氧化应激能力的调控。免疫印迹法检测Akt信号通路关键蛋白表达。结果:eoPE患者胎盘组织中SIRT3表达较对照组显著降低,SOD和GSH-Px水平明显降低,MDA水平明显升高。过表达SIRT3可促进HTR-8/SVneo细胞侵袭和迁移,显著缓解CoCl_(2)诱导的滋养细胞氧化应激。过表达SIRT3可显著上调Akt信号通路上的关键蛋白表达。结论:胎盘组织中SIRT3下调参与eoPE病理过程。SIRT3可能通过Akt信号通路促进滋养层细胞侵袭、迁移以及维持细胞氧化应激处于平衡状态。Objective:To detect SIRT3 expression in placental tissue from patients with early-onset preeclampsia(eoPE).To study the effects of SIRT3 on the function of trophoblast cell line HTR-8/SVneo cells.Methods:Western blot was used to detect the expression of SIRT3 in the placenta.The level of oxidative stress in placental tissue were detected by kits.Transiently transfected the plasmid was used to make HTR-8/SVneo cells overexpress SIRT3.The effects of SIRT3 on invasion and migration of HTR-8/SVneo cells were detected.The model of oxidative stress in trophoblasts was constructed through CoCl_(2)to study the regulation of SIRT3 on the ability of HTR-8/SVneo cells to resist oxidative stress.The key proteins on the Akt signaling pathway was observed using Western blot.Results:The expression of SIRT3 in placenta of eoPE was significantly lower than that in the normal control group.The SOD and GSH-Px levels were significantly decreased and the MDA level was significantly increased in placental tissue of eoPE patients.The up-regulated expression of SIRT3 can enhance the migration and invasion abilities of trophoblast cells.In addition,increased SIRT3 expression in HTR-8/SVneo cells significantly alleviated oxidative stress induced by CoCl_(2)in trophoblast cells.SIRT3 overexpression also significantly upregulated the expression of key proteins in the Akt signaling pathway.Conclusions:Down regulation of SIRT3 in placental tissue is involved in the pathological process of eoPE.SIRT3 may promote trophoblast cell invasion,migration,and maintain cellular oxidative stress in balanced state via Akt signaling pathway.

关 键 词：早发型子痫前期 SIRT3 滋养细胞 胎盘 

分 类 号：R714[医药卫生—妇产科学]