苗药细风轮菜的质量标准研究  

Study on quality standard for Miao medicine Clinopodium gracile

作　　者：杨转珍 杨永泓 种叶敏 蒋孟莲 张瑾 王珑静 龙飞[1] 李敏[1] YANG Zhuanzhen;YANG Yonghong;CHONG Yemin;JIANG Menglian;ZHANG Jin;WANG Longjing;LONG Fei;LI Min(School of Pharmacy/School of Modern Chinese Medicine Industry/State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院/现代中药产业学院/省部共建西南特色中药资源国家重点实验室,成都611137

出　　处：《中国药房》2023年第6期682-686,692,共6页China Pharmacy

基　　金：四川省药品监督管理局中药(民族药)标准提升项目(No.510201202102305)。

摘　　要：目的建立细风轮菜的质量标准。方法收集10批细风轮菜药材样品,对其进行外观性状、显微鉴别和薄层鉴别,并对其水分、总灰分、酸不溶性灰分、稀乙醇浸出物进行检查;采用高效液相色谱法测定药材中迷迭香酸的含量。结果细风轮菜药材茎纤细,呈方柱形,被白色绒毛,表面为灰绿色或绿褐色;茎横切面可见表皮细胞、非腺毛、皮层细胞等;粉末可见非腺毛、导管、木纤维、叶肉细胞等。薄层鉴别结果显示,供试品色谱中,在与醉鱼草皂苷Ⅳb对照品色谱相应的位置上,显相同颜色的斑点。10批样品的水分、总灰分、酸不溶性灰分、稀乙醇浸出物、迷迭香酸含量分别为8.69%~12.33%、5.96%~13.33%、0.14%~3.29%、18.57%~32.61%、0.35%~0.82%,平均值分别为10.10%、9.73%、1.06%、23.54%、0.56%。结论所建方法可用于细风轮菜药材的质量控制。初步拟定细风轮菜药材中水分不得过12.0%,总灰分不得过12.0%,酸不溶性灰分不得过1.5%,稀乙醇浸出物不得少于18.0%,迷迭香酸含量不得少于0.45%。OBJECTIVE To establish the quality standard of Clinopodium gracile.METHODS Ten batches of C.gracile were collected to perform appearance and property identification,microscopic identification and thin layer chromatography(TLC)identification.Moisture,total ash,acid-insoluble ash and dilute ethanol extract were detected,and the content of rosmarinic acid was determined by HPLC.RESULTS The stem of C.gracile was slender,square columnar,covered by white fluff,the surface was grayish green or greenish brown;epidermal cells,non-glandular hairs,cortical cells and so on were seen in the cross section of the stem.Non-glandular hairs,ducts,wood fibers,mesophyll cells and so on could be seen in the powder.Results of TLC identification showed that there were spots of the same color in the chromatographic position corresponding to the chromatographic position of buddlejasaponinⅣb control.The contents of water,total ash,acid-insoluble ash,dilute ethanol extract and rosmarinic acid in 10 batches of samples were 8.69%-12.33%,5.96%-13.33%,0.14%-3.29%,18.57%-32.61%,0.35%-0.82%,respectively.The average values were 10.10%,9.73%,1.06%,23.54%and 0.56%,respectively.CONCLUSIONS The established method can be used for quality control of C.gracile.It is preliminarily proposed that the ash content in the herb should not exceed 12.0%,the total ash content should not exceed 12.0%,the acid-insoluble ash content should not exceed 1.5%,the dilute ethanol extract should not be less than 18.0%,and the rosmarinic acid content should not be less than 0.45%.

关 键 词：细风轮菜 质量标准 醉鱼草皂苷Ⅳb 迷迭香酸 含量测定 

分 类 号：R917[医药卫生—药物分析学] R282[医药卫生—药学]