桑仁清肺口服液的质量标准提升研究  

Research on the Promotion of Sangrenqingfei Oral Liquid Quality Standard

作　　者：杜西翠 郭艳钦 刘格格 张趁 徐丹 李云波 李莹洁 朱秋艳 DU Xi-cui;GUO Yan-qin;LIU Ge-ge;ZHANG Chen;XU Dan;LI Yun-bo;LI Ying-jie;ZHU Qiu-yan(Hebei Jinkun Animal Medicine Co.,Ltd.,Xinji Hebei 052360,China;Hebei Veterinary Medicine Preparation Technology Innovation Center,Xinji Hebei 052360,China;Institute of veterinary drug control of Hebei Province,Shijiazhuang 050051,China;Bureau of Agriculture and Rural Affairs of the Laoting County,Tangshan,Hebei 063600,China)

机构地区：[1]河北锦坤动物药业有限公司,河北辛集052360 [2]河北省兽用中药制剂技术创新中心,河北辛集052360 [3]河北省兽药监察所,石家庄050051 [4]乐亭县农业农村局,河北唐山063600

出　　处：《中国兽药杂志》2023年第3期24-29,共6页Chinese Journal of Veterinary Drug

摘　　要：为了解决桑仁清肺口服液甘草鉴别斑点不清晰无法判定结果的问题,完善质量标准,采用HPLC法测定桑仁清肺口服液中的甘草酸含量,色谱条件为:Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);乙腈-0.1%磷酸(33∶67)为流动相;检测波长为250 nm。研究结果表明,甘草酸在19.09μg/mL~305.50μg/mL范围内具有良好的线性关系,其回归方程为y=7.17956 x-12.98810,R^(2)=0.99932,平均回收率为102.92%,RSD为0.86%。本方法简单准确重复性好,能更好地控制桑仁清肺口服液的质量,可作为桑仁清肺口服液的质量标准。In order to solve the problem that the spots were not clear and the result could not be determined,the content of glycyrrhizic acid in Sangrenqingfei Oral Liquid was determined by HPLC.Stationary phase were performed on Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5μm)and the mobile phase was acetonitrile and 0.1%phosphoric acid(33∶67),the detection wavelength was 250 nm.The results showed that glycyrrhizic acid had a good linear relationship when the garden in range of 19.09 to 305.50μg.Its regression equation was y=7.17956 X-12.98810 and R^(2)=0.99932.The average recovery was 102.92%and RSD was 0.86%.This method was simple,accurate and reproducible,which could better control the quality of the Sangrenqingfei Oral Liquid,and could be used as the quality standard of Sangrenqingfei Oral Liquid.

关 键 词：桑仁清肺口服液 HPLC 甘草酸 质量标准 

分 类 号：S859.79[农业科学—临床兽医学]