妊娠期糖尿病状态下小鼠棕色脂肪组织转录组学分析以及产热活性变化  

Analysis of the transcriptomics and the thermogenic activity changes of brown adipose tissue of pregnant mice with gesta-tional diabetes mellitus

作　　者：潘怡慧 肖文[2] 朱玉婷 殷亚东 刘岚[1] PAN Yihui;XIAO Wen;ZHU Yuting;YIN Yadong;LIU Lan(Women's Hospital affiliated to Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital),Nanjing,Jiangsu Province,210004;Jiangsu Second Normal University,Nanjing)

机构地区：[1]南京医科大学附属妇产医院,南京市妇幼保健院,210004 [2]江苏第二师范学院

出　　处：《中国计划生育学杂志》2023年第3期495-499,738,739,共7页Chinese Journal of Family Planning

基　　金：国家自然科学基金面上项目(82070879)。

摘　　要：目的:探讨妊娠期糖尿病(GDM)小鼠棕色脂肪组织(BAT)转录组学变化以及产热活性变化。方法:通过高通量测序技术分析6例孕晚期小鼠(正常妊娠对照组和GDM组各3例)BAT的转录组表达谱,以筛选差异表达基因,并进行基因本体(GO)和京都基因及基因组百科全书(KEGG)分析。采用免疫组织化学、蛋白免疫印迹和实时荧光定量PCR方法检测产热功能标志蛋白解偶联蛋白(UCP1)表达,评估GDM状态下小鼠BAT产热活性变化。结果:高通量测序共识别出372个差异表达基因,其中255个差异基因在GDM中上调表达,117个差异基因下调表达。GO和KEGG分析表明,这些差异基因的主要靶点与脂代谢异常、胰岛素信号转导等密切相关。与对照组相比,GDM组BAT出现脂滴增大、质地疏松改变,UCP1蛋白及基因表达降低。结论:GDM组BAT与正常妊娠者转录组图谱存在显著差异,且GDM组BAT产热活性下降,可能引起糖脂代谢紊乱及胰岛素抵抗。Objective:To investigate the transcriptomics change of brown adipose tissue(BAT)of pregnant mice with gestational diabetes mellitus(GDM),and to study its influence on the thermogenic activity changes.Methods:The transcriptome expression profiles of 6 pregnant mice were analyzed by high-throughput sequencing,which included 3 normal pregnant mice in group A and 3 pregnant mice with GDM in group B.The differentially expressed genes of the mice were screening.The gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were performed.The expression of the uncoupling protein 1(UCP1)of marker protein of the mice was detected by immuno-histochemistry,Western blot,and real-time PCR in order to evaluate the thermogenic activity of BAT of the mice with GDM.Results:The high-throughput sequencing had identified 372 dfrentially expressed genes,of which,255 genes of the mice with GDM were down-regulated and 117 genes were up-regulated.GO and KEGG analysis showed that the main targets of these differential genes of the mice were closely related to the abnormal lipid metabolism and the insulin signal transduction.Compared with those of the mice in group A,BAT of the mice in group B showed the increased lipid droplets,the loose texture,and the decreased UCP1 protein and gene expressions.Conclusion:The transcriptome profiles in BAT of the mice with GDM are significantly different from that of the mice with normal pregnancy.The thermogenic activity of the mice with GDM decreases,which may cause the glucolipid metabolism and insulin resistance disorders of these mice.

关 键 词：妊娠期糖尿病 小鼠 棕色脂肪组织 转录组测序分析 产热活性 

分 类 号：R714.256[医药卫生—妇产科学]