海洋菌群厌氧生物降解环己甲酸特性研究  

Study of anaerobic biodegradation characteristics of cyclohexanecarboxylic acid by marine consortium

作　　者：昝帅君 金媛 樊景凤[2] 杜苗苗 王竞[1] ZAN Shuaijun;JIN Yuan;FAN Jingfeng;DU Miaomiao;WANG Jing(School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China;National Marine Environment Monitoring Center,Dalian 116023,China)

机构地区：[1]大连理工大学环境学院,辽宁大连116024 [2]国家海洋环境监测中心,辽宁大连116023

出　　处：《大连理工大学学报》2023年第2期135-142,共8页Journal of Dalian University of Technology

基　　金：国家自然科学基金资助项目(21876018,42177375)。

摘　　要：环烷酸是一类具有较强毒性的环境新污染物.目前,环烷酸生物降解研究主要集中在陆地和好氧环境,而海洋环境中环烷酸的厌氧生物降解鲜有报道.利用富集的海洋微生物菌群,考察了环己甲酸在不同条件下的厌氧生物降解特性,并通过16S高通量测序对微生物群落结构和功能进行了分析.结果表明:共底物葡萄糖和乙酸钠会抑制环己甲酸的厌氧生物降解;硝酸钠(200 mg/L)可提高环己甲酸厌氧生物降解速率约70%,并在100 mg/L亚微米磁铁共存下进一步提高反硝化降解效率约44.5%,降解过程均符合准一级动力学反应.变形菌门、厚壁菌门和拟杆菌门为优势菌门;弧菌属、交替单胞菌属和梭菌属为优势菌属.此外,细胞膜转运及能量、碳水化合物和氨基酸代谢通路有显著富集.研究拓展了环烷酸在海洋环境中的生物转化行为,并为海洋环境中环烷酸厌氧生物修复提供了启示.Naphthenic acids(NAs) are a new class of environmental pollutants with strong toxicity. At present, the biodegradation of NAs is mainly concentrated in the terrestrial environment, and most of them are aerobic degradation, while the anaerobic biodegradation of NAs in the marine environment has rarely been reported. The enriched marine consortium is used to investigate the anaerobic biodegradation characteristics of cyclohexanecarboxylic acid(CHCA) under different conditions, and the microbial community structure and function are analyzed by 16S high-throughput sequencing. The results show that the glucose and sodium acetate of co-substrates can inhibit the anaerobic biodegradation of CHCA, the sodium nitrate(200 mg/L) can increase the anaerobic biodegradation rate of CHCA by about 70%, and the denitrification degradation efficiency is further increased by about 44.5% under the coexistence of 100 mg/L submicron magnets, and the anaerobic biodegradation of CHCA follows the pseudo-first-order kinetic reaction. The Proteobacteria, Firmicutes and Bacteroidetes are the dominant bacterial phyla;Vibrio, Alteromonas and Clostridium are the dominant bacterial genus. In addition, pathways of cell membrane transport and metabolism of energy, carbohydrate and amino acid are significantly enriched during the CHCA degradation. The study expands the biotransformation behavior of NAs and provides enlightenment for the anaerobic bioremediation of NAs in the marine environment.

关 键 词：环烷酸 海洋环境 厌氧降解 电子受体 群落结构 

分 类 号：X53[环境科学与工程—环境工程]