野生鸟类趾爪携带细菌多样性及其潜在致病性种群分析  

Species diversity and potential pathogenicity of bacteria carried by wild birds’claws in Yunnan province

作　　者：姜林 邓巍 张淑霞 杨晓燕 JIANG Lin;DENG Wei;ZHANG Shu-xia(Institute of Eastern-Himalaya Biodiversity Research,Dali University,Dali,Yunnan Province 671003,China;不详)

机构地区：[1]大理大学东喜玛拉雅研究院,云南671003 [2]中国三江并流区域生物多样性协同创新中心 [3]三江并流区域生物多样性保护与利用云南省创新团队

出　　处：《中国公共卫生》2023年第1期36-41,共6页Chinese Journal of Public Health

基　　金：第二次青藏高原综合科学考察研究资助(2019QZKK0402);云南省教育厅科学研究基金(2021Y390)。

摘　　要：目的初步调查野生鸟类趾爪携带细菌的多样性及潜在致病性种群,从生态学角度明确开展野生鸟类携带致病性细菌监测的必要性。方法采集野生鸟类趾爪样品12份,采用基于扩增子序列变体(amplicon sequence variants,ASV)的高通量测序技术分析不同鸟类趾爪携带的细菌多样性及致病性细菌的组成。结果野生鸟类趾爪可携带其生境中的优势细菌,不同鸟类携带的细菌及组成相似度与鸟类生活习性相关。在属和种水平,野生鸟类携带的致病菌总占比均较高,分别为81.25%和75.00%,其中,林鸟携带有更多种类的致病细菌;水鸟携带的致病菌除少量为植物病原菌外,其他均为对人致病的细菌。结论野生鸟类趾爪会携带其生境中的致病细菌,对不同类型野生鸟类携带的病原微生物的监测需要纳入公共卫生部门的日常监测范围;同时野生鸟类趾爪可作为重点样品采集部位。Objective To investigate species diversity and potential pathogenicity of bacteria carried by wild birds’claws preliminarily for clarifying the necessity of monitoring pathogenic bacteria carried by wild birds.Methods Toe/claw swab specimens were collected from 12 kinds of wild forest/aquatic birds for isolation of bacteria.Amplicon sequence variants(ASV)-based high-throughput sequencing was used to analyze bacterial species composition and pathogenic bacteria isolated.Results A large number of bacteria dominant in the wild birds’habitats were detected and the bacterial spectrum and its similarity for the bacteria detected in the wild birds of different species were associated with the birds’living habitats.Of all the detected bacteria at genus and species levels,81.25%and 75.00%were pathogenic bacteria,with more kinds of pathogenic bacteria detected in the forest birds and all as human pathogenic bacteria except for a small amount of plant pathogenic bacteria in the aquatic birds.Conclusion Wild birds’toes/claws could carry pathogenic bacteria from their habitats and the detection of pathogenic microorganisms carried by different types of wild birds needs to be included in the scope of routine monitoring for public health.

关 键 词：野生鸟类 公共卫生 高通量测序技术 传染病 致病性细菌 

分 类 号：Q938.1[生物学—微生物学]