辽东地区胡桃楸人工林根际和非根际土壤细菌多样性  

Bacterial Diversity in Rhizosphere and Non-rhizosphere Soil of the Juglans mandshurica Demonstration Forest in Eastern Liaoning

作　　者：吴光伟 傅靖棋 赫亮亮 范新蕊 苏治鑫 朱瑞 孙建文 张丽杰[1] WU Guang-wei;FU Jing-qi;HE Liang-liang;FAN Xin-rui;SU Zhi-xin;ZHU Rui;SUN Jian-wen;ZHANG Li-jie(College of Forestry/Key Laboratoryof Forest Tree Genetics,Breeding and Cultivation of Liaoning Province,Shenyang Agricultural University,Shenyang 110161,China;Liaoning Inspection,Examination&Certification Centre,Shenyang 110032,China;Three Stone Forest Grounds of Fushun County Liaon-ing Province,Fushun Liaoning 113108,China;Fushun Forestry Development Service Center,Fushun Liaoning 113006,China)

机构地区：[1]沈阳农业大学林学院/辽宁省林木遗传育种与培育重点实验室,沈阳110161 [2]辽宁省检验检测认证中心,沈阳110032 [3]抚顺县三块石林场,辽宁抚顺113108 [4]抚顺市林业发展服务中心,辽宁抚顺113006

出　　处：《沈阳农业大学学报》2022年第6期723-730,共8页Journal of Shenyang Agricultural University

基　　金：“十三五”国家重点研发计划项目(2017YFD0600600);辽宁省教育厅重点攻关项目(LSNZD201905)。

摘　　要：对胡桃楸人工林土壤细菌群落结构特征和土壤理化性质进行研究,为培育速生优质胡桃楸大径材林木提供理论依据。通过Illumina NovaSeq高通量测序分析人工示范林胡桃楸根际和非根际土壤细菌多样性及群落结构与土壤理化因子的相关性,结果表明:人工林地根际土壤呈弱酸性,非根际土壤为酸性,根际土壤pH极显著高于非根际土壤(p<0.01);根际土壤全碳、全氮含量显著高于非根际土壤(p<0.05);根际土壤全磷、有效磷含量极显著高于非根际土壤(p<0.01);根际土壤速效氮含量低于非根际土壤,但二者没有显著性差异。胡桃楸人工林土壤微生物中细菌多样性丰富,测序共得到168657条有效细菌序列,共鉴别出36门,106纲,242目,354科,630属。分析发现在门分类水平上根际和非根际土壤优势细菌均为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)和酸杆菌门(Acidobacteriota)。在细菌目水平上,根际和非根际土壤优势细菌分别为:盖勒氏菌目(Gaiellales)、根瘤菌目(Rhizobiales)、土壤红杆菌目(Solirubrobacterales)和维氏细菌目(Vicinamibacterales)等。细菌多样性分析表明,根际土壤细菌香农指数、ACE指数显著高于非根际土壤(p<0.05);Chao1指数极显著高于非根际土壤(p<0.01)。土壤微生物中放线菌门和变形菌门与土壤pH、全磷含量、全氮含量和有效磷含量呈正相关,且与土壤速效氮含量呈负相关;酸杆菌门与土壤理化性质呈负相关(土壤速效氮含量除外);绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)和粘球菌门(Myxococcota)与土壤pH、全碳含量和全氮含量呈正相关;甲基微菌门(Methylomirabilota)与土壤pH呈显著负相关(r=-0.916,p<0.05),与土壤有效磷含量呈极显著负相关(r=-0.925,p<0.01)。The study on the characteristics of soil bacterial community structure and soil physical and chemical properties of Juglans mandshurica plantation would provide a theoretical basis for cultivating fast-growing,high-quality and big diameter timber trees.In this study,Illumina NovaSeq high-throughput sequencing was used to analyze the correlation between bacterial diversity,community structure and soil physical and chemical factors in rhizosphere and non rhizosphere soil of J.mandshurica in the artificial demonstration forest.The results showed that the rhizosphere soil of the plantation was weakly acidic,while the non-rhizosphere soil was acidic,and the pH of rhizosphere soil was extremely significant higher than the non-rhizosphere soil(p<0.01);The contents of total carbon and nitrogen in rhizosphere soil were significantly higher than those in non-rhizosphere soil(p<0.05);The contents of total phosphorus and available phosphorus in rhizosphere soil were extremely significant higher than those in non-rhizosphere soil(p<0.01);The available nitrogen content in rhizosphere soil was lower than that in non-rhizosphere soil,but there was no significant difference between them.The diversity of bacteria in soil microorganisms of J.mandshurica plantation was rich.A total of 168657 effective bacterial sequences were obtained by sequencing,and 36 phyla,106 classes,242 orders,354 families and 630 genera were identified.The analysis showed that the dominant bacteria in rhizosphere and non-rhizosphere soil were Actinobaciota,Proteobactera and Acidobacter at the phyla classification level;At the level of bacteria order,the dominant bacteria in rhizosphere and non-rhizosphere soil were Gaillares,Rhizobiales,Solirubrobacteriales and Vicinamibacteria,etc.Bacterial diversity analysis showed that Shannon index,ACE index extremely significant of bacteria in rhizosphere soil were significantly higher than those in non-rhizosphere soil(p<0.05);The Chao1 index was significantly higher than that of non-rhizosphere(p<0.01).The actinomycet

关 键 词：胡桃楸 根际和非根际 细菌多样性 群落结构 

分 类 号：S792.132[农业科学—林木遗传育种]