外源硫化氢调控盐碱胁迫下裸燕麦叶片糖和酚酸代谢反应  

Exogenous hydrogen sulfide modulates metabolic responses of sugar and phenolic acid in naked oat leaves under saline-alkali stress

作　　者：刘建新[1] 刘瑞瑞[1] 刘秀丽[1] 贾海燕[1] 卜婷[1] 李娜[1] LIU Jianxin;LIU Ruirui;LIU Xiuli;JIA Haiyan;BU Ting;LI Na(Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration in East Gansu/College of Life Sciences and Technology,Longdong University,Qingyang 745000,China)

机构地区：[1]甘肃省陇东生物资源保护利用与生态修复重点实验室/陇东学院生命科学与技术学院,庆阳745000

出　　处：《中国生态农业学报（中英文）》2023年第3期463-477,共15页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(31960375);甘肃省自然科学基金项目(20JR5RA491)资助。

摘　　要：为明确硫化氢信号对盐碱胁迫下植物代谢组的调控作用,揭示其增强植物耐盐碱性的机理,以裸燕麦(Avena nuda L.)为材料进行盆栽土培试验,设置不添加盐碱和添加3.00 g·kg^(-1)盐碱(摩尔比NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)∶NaHCO_(3)=12∶8∶1∶9)与裸燕麦抽穗期叶面喷施蒸馏水和50μmol·L^(-1)硫化氢供体硫氢化钠溶液交叉共4组处理,利用超高效液相色谱-串联质谱技术结合正交偏最小二乘判别分析方法,研究外源硫化氢对盐碱胁迫下裸燕麦叶片糖分解代谢产物水平、氧化还原平衡、酚酸含量和产量性状的影响。结果表明:1)非盐碱条件下,喷施硫氢化钠对裸燕麦叶片还原型谷胱甘肽/氧化型谷胱甘肽、还原型辅酶Ⅱ/氧化型辅酶Ⅱ、腺苷三磷酸含量和产量性状的影响微弱,但显著上调柠檬酸、琥珀酸和6-磷酸葡萄糖酯含量,显著下调葡萄糖-6-磷酸、丙酮酸、乳酸、α-酮戊二酸、谷氨酸、天冬酰胺、赤藓糖-4-磷酸、景天庚酮糖-7-磷酸含量;盐碱胁迫导致裸燕麦叶片葡萄糖、葡萄糖-6-磷酸、果糖-6-磷酸、果糖-1,6-二磷酸、3-磷酸甘油醛、3-磷酸甘油酸、丙酮酸、乳酸、α-酮戊二酸、谷氨酸、谷氨酰胺、天冬酰胺、赤藓糖-4-磷酸、景天庚酮糖-7-磷酸、核糖-5-磷酸等糖酵解、三羧酸循环和戊糖磷酸途径中间代谢物及还原型谷胱甘肽、氧化型谷胱甘肽、还原型辅酶Ⅱ、氧化型辅酶Ⅱ含量显著降低,而还原型谷胱甘肽/氧化型谷胱甘肽显著提高;喷施硫氢化钠显著提高盐碱胁迫下裸燕麦叶片葡萄糖、果糖-6-磷酸、3-磷酸甘油酸、乳酸、α-酮戊二酸、延胡索酸、苹果酸、谷氨酰胺、6-磷酸葡萄糖酯、景天庚酮糖-7-磷酸含量,显著降低天冬酰胺含量。2)喷施硫氢化钠显著下调非盐碱条件下裸燕麦叶片反式肉桂酸和丁香醛含量;盐碱胁迫导致裸燕麦叶片反式肉桂酸含量显著降低,而苯甲酸、�In order to clarify the regulatory effect of hydrogen sulfide signaling on the plant metabolome under saline-alkali stress and to reveal its mechanism of enhancing plant saline-alkali tolerance,a pot experiment was conducted with naked oat(Avena nude)as the material.Four treatments were applied to potted naked oat plants in a 2×2 factorial combination,including 0 or 3.00 g·kg^(-1)salinealkali(molar ratio of NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)∶NaHCO_(3)at 12∶8∶1∶9)added to the potting soil and spraying with distilled water or 50μmol·L^(-1)sodium hydrosulfide(a hydrogen sulfide donor)on leaves at the heading stage.The effects of exogenous hydrogen sulfide on glycolytic metabolite levels,redox balance,and phenolic acid content in leaves and on the yield traits of naked oats under the four treatments were investigated using ultra-performance liquid chromatography-tandem mass spectrometry technology combined with orthogonal partial least squares discriminant analysis.Under non-saline-alkali conditions,sodium hydrosulfide application did not have a significant effect on the ratios of(reduced glutathione)/(oxidized glutathione)and(reduced coenzymeⅡ)/(oxidized coenzymeⅡ),adenosine triphosphate content in leaves,and yield traits of naked oats;however,the levels of citrate,succinate,and 6-phosphogluconolactone were significantly upregulated and those of glucose-6-phosphate,pyruvate,lactate,α-ketoglutaric acid,glutamate,asparagine,erythrose-4-phosphate,and sedoheptulose-7-phosphate were significantly downregulated in the leaves.Salinealkali stress significantly reduced the levels of glucose,glucose-6-phosphate,fructose-6-phosphate,fructose-1,6-diphosphate,3-phosphate glyceraldehydes,3-phosphoglyceric acid,pyruvate,lactate,α-ketoglutaric acid,glutamate,glutamine,asparagine,erythrose-4-phosphate,sedoheptulose-7-phosphate,ribose-5-phosphate,reduced glutathione,oxidized glutathione,reduced coenzymeⅡ,and oxidized coenzymeⅡin the leaves of naked oats;whereas the ratio of reduced glutathione to oxidized glutat

关 键 词：硫化氢 盐碱胁迫 裸燕麦 糖代谢 酚酸 

分 类 号：S512.6[农业科学—作物学]