LC-PDA测定槟榔、焦槟榔、大腹皮中4种生物碱的含量  

Determination of Four Alkaloids Components in Arecae Semen,Arecae Semen Tostum and Arecae Pericarpium by LC-PDA

作　　者：侯倩煜 符礼顺 梁焕宴 周亚奎[1] 杨新全[1] 赵祥升[1] HOU Qianyu;FU Lishun;LIANG Huanyan;ZHOU Yakui;YANG Xinquan;ZHAO Xiangsheng(Hainan Branch Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Haikou 570311,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海口570311

出　　处：《中国现代应用药学》2023年第4期443-447,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(82074130);海南省重点研发计划项目(ZDYF2020183,ZDYF2023SHFZ141);海南省基础与应用基础研究计划高层次人才项目(2019RC342);中国医学科学院医学与健康科技创新工程(2021-I2M-1-032)。

摘　　要：目的建立槟榔、焦槟榔、大腹皮中槟榔碱、槟榔次碱、去甲槟榔碱、去甲槟榔次碱含量的LC-PDA测定方法。方法样品加氨水浸润,甲醇超声提取。ZORBAX 300-SCX色谱柱(150 mm×4.6 mm,5μm)分离,流动相A为乙腈,流动相B为0.2%甲酸水(氨水调节pH=3.8),梯度洗脱,流速为0.5 mL·min^(-1),柱温为30℃,进样量为5μL,检测波长为225 nm。结果槟榔碱、槟榔次碱、去甲槟榔碱、去甲槟榔次碱分别在2.98~745.0,2.60~650.0,1.19~595.0,1.22~610.0μg·mL^(-1)内与峰面积呈良好的线性关系(r^(2)>0.9990)。槟榔、焦槟榔、大腹皮中槟榔碱、槟榔次碱、去甲槟榔碱、去甲槟榔次碱的平均加样回收率(n=6)均在95.0%~105.0%,RSD<2.50%。槟榔和其炮制品中生物碱含量差异显著。结论该方法快捷、灵敏、准确、可靠,可用于槟榔、焦槟榔、大腹皮中4种生物碱的含量测定,为槟榔及其饮片的质量控制提供了参考。OBJECTIVE To establish an LC-PDA method for determination of arecoline,arecaidine,guvacoline and guvacine in Arecae Semen,Arecae Semen Tostum and Arecae Pericarpium.METHODS The sample was soaked with ammonia,and extracted with methanol by ultrasonic extraction.The target compounds were separated on a ZORBAX 300-SCX column(150 mm×4.6 mm,5μm)with a gradient elution of acetonitrile(A)-0.2%acetic acid(B)solution(adjusted to pH=3.8 with ammonium)at the flow rate of 0.5 mL·min^(-1).The column temperature was 30℃,the injection volume was 5μL,and the detection wavelength was set at 225 nm.RESULTS Arecoline,arecaidine,guvacoline and guvacine showed a good linear relationship with the peak area within 2.98–745.0,2.60–650.0,1.19–595.0,1.22–610.0μg·mL^(-1),respectively(r^(2)>0.9990).The average recoveries of arecoline,arecaidine,guvacoline and guvacine in Arecae Semen,Arecae Semen Tostum and Arecae Pericarpium were ranged from 95.0%to 105.0%,with RSD<2.50%(n=6).The content of alkaloids in Arecae Semen and its processed products was significantly different.CONCLUSION The proposed method is rapid,sensitive,accurate,reliable,which can be used for the determination of four alkaloids in Arecae Semen,Arecae Semen Tostum and Arecae Pericarpium.

关 键 词：槟榔 焦槟榔 大腹皮 槟榔碱 槟榔次碱 去甲槟榔碱 去甲槟榔次碱 液相色谱 

分 类 号：R917[医药卫生—药物分析学]