大麦根系响应湿害胁迫的转录组分析  

Transcriptome Analysis of Barley Root in Response to Waterlogging Stress

作　　者：栾海业 王春吉 陈昌宇 王鸿 杨力 张英虎[2] 乔海龙[2] 杨红燕 沈会权[2] 臧慧 LUAN Haiye;WANG Chunji;CHEN Changyu;WANG Hong;YANG Li;ZHANG Yinghu;QIAO Hailong;YANG Hongyan;SHEN Huiquan;ZANG Hui(Yancheng Teachers University,Yancheng,Jiangsu 224002,China;Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng,Jiangsu 224002,China;Yancheng Grain and Oil Crop Technical Gruidance Station,Yancheng,Jiangsu 224002,China)

机构地区：[1]盐城师范学院,江苏盐城224002 [2]江苏沿海地区农业科学研究所,江苏盐城224002 [3]盐城市粮油作物技术指导站,江苏盐城224002

出　　处：《麦类作物学报》2023年第2期150-156,共7页Journal of Triticeae Crops

基　　金：啤酒大麦产量与质量协同技术推广计划项目(2020-ZYXT-04-2);江苏省自然科学基金项目(BK20201215);国家大麦青稞产业技术体系专项(CARS-05);江苏省作物基因组学和分子育种重点实验室开放课题项目(PL202102);江苏现代农业(特粮特经)产业技术体系啤酒大麦创新团队项目(JATS[2021]228)。

摘　　要：湿害是影响大麦产量和品质的主要非生物胁迫之一。为进一步明确大麦响应湿害胁迫的应答机理,本研究以耐湿大麦品种泰兴9425为材料,利用高通量转录组测序(RNA-seq)技术,比较不同湿害胁迫时间下的基因表达差异。结果表明,湿害胁迫12 h后获得1436个差异表达基因,而胁迫48 h后获得2090个差异表达基因,其中共同上调表达基因286个。GO富集分析发现,涉及代谢过程、细胞膜、催化活性等的差异表达基因占比最多。KEGG富集分析发现,差异表达基因主要富集于糖酵解、类黄酮生物合成、乙烯合成等代谢通路。对7个耐湿相关的候选基因进行qRT-PCR分析,发现差异表达基因的表达量变化趋势与RNA-seq结果一致。Waterlogging is one of the major abiotic stresses that affects barley yield and quality.In order to further clarify the mechanism of barley response to waterlogging stress,Taixing 9425,a waterlogging tolerant barley variety,was used to compare the gene expression differences under different stress time by high-throughput transcriptomic sequencing analysis.The results showed that a total of 1436 differentially expressed genes(DEGs)were detected under waterlogging stress treatment for 12 h,while 2090 DEGs were detected under waterlogging stress treatment for 48 h,including 286 DEGs both up-regulated in 12 h and in 48 h.GO enrichment analysis showed that the functions of DEGs were mainly referred to metabolic process,cell membrane,catalytic activity,etc.KEGG enrichment analysis showed that DEGs were enriched in glycolysis and fermentation,flavonoid and ethylene biosynthesis,etc.We validated the expression profiles of seven candidate genes by qRT-PCR,and found the results were consistent with the RNA-seq results.

关 键 词：大麦 湿害胁迫 转录组 差异表达基因 

分 类 号：S512.3[农业科学—作物学] S330